CLASP2 sirna

CLASP2 siRNA (Human)

Cat. Num. AAA8214942

• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CLASP2; CLASP2 siRNA (Human); KIAA0627; CLIP-associating protein 2; Cytoplasmic linker-associated protein 2; Protein Orbit homolog 2; hOrbit2; CLASP2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: CLASP2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1294

• Applicable Applications for CLASP2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human CLASP2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CLASP2 sirna

siRNA to inhibit CLASP2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 333440451
• NCBI GeneID: 23122
• NCBI Accession #: NP_001193973.1
• NCBI GenBank Nucleotide #: NM_001207044.1
• UniProt Accession #: O75122; Q7L8F6; Q8N6R6; Q9BQT3; Q9BQT4; Q9H7A3; Q9NSZ2; B2RTR1; F5H604
• Molecular Weight: 165,924 Da
• NCBI Official Full Name: CLIP-associating protein 2 isoform 2
• NCBI Official Synonym Full Names: cytoplasmic linker associated protein 2
• NCBI Official Symbol: CLASP2
• NCBI Protein Information: CLIP-associating protein 2
• UniProt Protein Name: CLIP-associating protein 2
• Protein Family: CLIP-associating protein
• UniProt Gene Name: CLASP2
• UniProt Synonym Gene Names: KIAA0627; hOrbit2
• UniProt Entry Name: CLAP2_HUMAN

Uniprot Description

CLASP2: Microtubule plus-end tracking protein that promotes the stabilization of dynamic microtubules. Involved in the nucleation of noncentrosomal microtubules originating from the trans-Golgi network (TGN). Required for the polarization of the cytoplasmic microtubule arrays in migrating cells towards the leading edge of the cell. May act at the cell cortex to enhance the frequency of rescue of depolymerizing microtubules by attaching their plus-ends to cortical platforms composed of ERC1 and PHLDB2. This cortical microtubule stabilizing activity is regulated at least in part by phosphatidylinositol 3-kinase signaling. Also performs a similar stabilizing function at the kinetochore which is essential for the bipolar alignment of chromosomes on the mitotic spindle. Acts as a mediator of ERBB2-dependent stabilization of microtubules at the cell cortex. Belongs to the CLASP family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal

Chromosomal Location of Human Ortholog: 3p22.3

Cellular Component: kinetochore microtubule; Golgi apparatus; microtubule; membrane; cytoplasmic microtubule; cytoplasm; plasma membrane; microtubule organizing center; trans-Golgi network; cell cortex; cytosol

Molecular Function: microtubule plus-end binding; protein binding; galactoside 2-alpha-L-fucosyltransferase activity

Biological Process: axon guidance; mitosis; microtubule organizing center organization and biogenesis; regulation of microtubule-based process; cell division; negative regulation of microtubule depolymerization; establishment and/or maintenance of cell polarity; mitotic cell cycle; microtubule nucleation; regulation of microtubule polymerization or depolymerization