CHMP4A sirna

CHMP4A siRNA (Human)

Cat. Num. AAA8234043

• Gene Names: CHMP4A; SNF7; CHMP4; SHAX2; CHMP4B; SNF7-1; VPS32A; HSPC134; VPS32-1; C14orf123
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CHMP4A; CHMP4A siRNA (Human); C14orf123; SHAX2; Charged multivesicular body protein 4a; Chromatin-modifying protein 4a; CHMP4a; SNF7 homolog associated with Alix-2; SNF7-1; hSnf-1; Vacuolar protein sorting-associated protein 32-1; Vps32-1; hVps32-1; CHMP4A sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: CHMP4A siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 265

• Applicable Applications for CHMP4A sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human CHMP4A gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CHMP4A sirna

siRNA to inhibit CHMP4A expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 40548422
• NCBI GeneID: 29082
• NCBI Accession #: NP_054888.2
• NCBI GenBank Nucleotide #: NM_014169.3
• UniProt Accession #: Q9BY43; Q14D22; Q32Q79; Q86SZ8; Q96QJ9; Q9P026
• Molecular Weight: 29,804 Da
• NCBI Official Full Name: charged multivesicular body protein 4a
• NCBI Official Synonym Full Names: charged multivesicular body protein 4A
• NCBI Official Symbol: CHMP4A
• NCBI Official Synonym Symbols: SNF7; CHMP4; SHAX2; CHMP4B; SNF7-1; VPS32A; HSPC134; VPS32-1; C14orf123
• NCBI Protein Information: charged multivesicular body protein 4a
• UniProt Protein Name: Charged multivesicular body protein 4a
• Protein Family: Charged multivesicular body protein
• UniProt Gene Name: CHMP4A
• UniProt Synonym Gene Names: C14orf123; SHAX2; CHMP4a; hSnf-1; Vps32-1; hVps32-1
• UniProt Entry Name: CHM4A_HUMAN

NCBI Description

CHMP4A belongs to the chromatin-modifying protein/charged multivesicular body protein (CHMP) family. These proteins are components of ESCRT-III (endosomal sorting complex required for transport III), a complex involved in degradation of surface receptor proteins and formation of endocytic multivesicular bodies (MVBs). Some CHMPs have both nuclear and cytoplasmic/vesicular distributions, and one such CHMP, CHMP1A (MIM 164010), is required for both MVB formation and regulation of cell cycle progression (Tsang et al., 2006 [PubMed 16730941]).[supplied by OMIM, Mar 2008]

Uniprot Description

CHMP4A: Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. When overexpressed, membrane-assembled circular arrays of CHMP4A filaments can promote or stabilize negative curvature and outward budding. Via its interaction with PDCD6IP involved in HIV-1 p6- and p9-dependent virus release. Belongs to the SNF7 family.

Chromosomal Location of Human Ortholog: 14q12

Cellular Component: internal side of plasma membrane; membrane coat; cytoplasmic vesicle membrane; late endosome membrane; cytoplasm; plasma membrane; midbody; cytosol; nucleus; endosome

Molecular Function: identical protein binding; protein binding; protein homodimerization activity; lipid binding; ATPase binding

Biological Process: protein polymerization; cell separation during cytokinesis; viral reproduction; vacuolar transport; membrane invagination; viral infectious cycle; posttranslational protein targeting to membrane; endosome transport; mitotic metaphase plate congression; protein homooligomerization; nuclear organization and biogenesis; membrane budding