CHMP1A sirna

CHMP1A siRNA (Human)

Cat. Num. AAA8237609

• Gene Names: CHMP1A; PCH8; CHMP1; PRSM1; PCOLN3; VPS46A; VPS46-1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CHMP1A; CHMP1A siRNA (Human); CHMP1; KIAA0047; PCOLN3; PRSM1; Charged multivesicular body protein 1a; Chromatin-modifying protein 1a; CHMP1a; Vacuolar protein sorting-associated protein 46-1; Vps46-1; hVps46-1; CHMP1A sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: CHMP1A siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 68

• Applicable Applications for CHMP1A sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human CHMP1A gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CHMP1A sirna

siRNA to inhibit CHMP1A expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 103485496
• NCBI GeneID: 5119
• NCBI Accession #: NP_002759.2
• NCBI GenBank Nucleotide #: NM_002768.4
• UniProt Accession #: Q9HD42; Q14468; Q15779; Q96G31; A2RU09
• Molecular Weight: 7,363 Da
• NCBI Official Full Name: charged multivesicular body protein 1a isoform 2
• NCBI Official Synonym Full Names: charged multivesicular body protein 1A
• NCBI Official Symbol: CHMP1A
• NCBI Official Synonym Symbols: PCH8; CHMP1; PRSM1; PCOLN3; VPS46A; VPS46-1
• NCBI Protein Information: charged multivesicular body protein 1a
• UniProt Protein Name: Charged multivesicular body protein 1a
• Protein Family: Charged multivesicular body protein
• UniProt Gene Name: CHMP1A
• UniProt Synonym Gene Names: CHMP1a; Vps46-1; hVps46-1
• UniProt Entry Name: CHM1A_HUMAN

NCBI Description

This gene encodes a member of the CHMP/Chmp family of proteins which are involved in multivesicular body sorting of proteins to the interiors of lysosomes. The initial prediction of the protein sequence encoded by this gene suggested that the encoded protein was a metallopeptidase. The nomenclature has been updated recently to reflect the correct biological function of this encoded protein. Several transcripts encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2012]

Uniprot Description

CHMP1A: Probable peripherally associated component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT- III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. Involved in cytokinesis. Involved in recruiting VPS4A and/or VPS4B to the midbody of dividing cells. May also be involved in chromosome condensation. Targets the Polycomb group (PcG) protein BMI1/PCGF4 to regions of condensed chromatin. May play a role in stable cell cycle progression and in PcG gene silencing. Belongs to the SNF7 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Protease; Cell cycle regulation

Chromosomal Location of Human Ortholog: 16q24.3

Cellular Component: condensed nuclear chromosome; nuclear matrix; early endosome; endomembrane system; microtubule organizing center

Molecular Function: protein domain specific binding; protein binding; protein homodimerization activity; zinc ion binding; metallopeptidase activity

Biological Process: vesicle-mediated transport; protein transport; cell separation during cytokinesis; transcription, DNA-dependent; negative regulation of transcription by glucose; mitotic chromosome condensation; vacuolar transport; cytokinesis; gene silencing; negative regulation of transcription, DNA-dependent; proteolysis; mitotic metaphase plate congression; nuclear organization and biogenesis

Disease: Pontocerebellar Hypoplasia, Type 8