CEP250 sirna

CEP250 siRNA (Human)

Cat. Num. AAA8238972

• Gene Names: CEP250; CEP2; CNAP1; C-NAP1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CEP250; CEP250 siRNA (Human); CEP2; CNAP1; Centrosome-associated protein CEP250; 250 kDa centrosomal protein; Cep250; Centrosomal Nek2-associated protein 1; C-Nap1; Centrosomal protein 2; CEP250 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: CEP250 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 2442

• Applicable Applications for CEP250 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human CEP250 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CEP250 sirna

siRNA to inhibit CEP250 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 21735548
• NCBI GeneID: 11190
• NCBI Accession #: NP_009117.2
• NCBI GenBank Nucleotide #: NM_007186.4
• UniProt Accession #: Q9BV73; O14812; O60588; Q9H450; E1P5Q3
• Molecular Weight: 274,380 Da
• NCBI Official Full Name: centrosome-associated protein CEP250
• NCBI Official Synonym Full Names: centrosomal protein 250kDa
• NCBI Official Symbol: CEP250
• NCBI Official Synonym Symbols: CEP2; CNAP1; C-NAP1
• NCBI Protein Information: centrosome-associated protein CEP250
• UniProt Protein Name: Centrosome-associated protein CEP250
• Protein Family: Centrosome-associated protein
• UniProt Gene Name: CEP250
• UniProt Synonym Gene Names: CEP2; CNAP1; Cep250; C-Nap1
• UniProt Entry Name: CP250_HUMAN

NCBI Description

This gene encodes a core centrosomal protein required for centriole-centriole cohesion during interphase of the cell cycle. The encoded protein dissociates from the centrosomes when parental centrioles separate at the beginning of mitosis. The protein associates with and is phosphorylated by NIMA-related kinase 2, which is also associated with the centrosome. [provided by RefSeq, Jun 2009]

Uniprot Description

CEP250: Probably plays an important role in centrosome cohesion during interphase. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation

Chromosomal Location of Human Ortholog: 20q11.22

Cellular Component: centriole; centrosome; protein complex; perinuclear region of cytoplasm; microtubule organizing center; spindle pole centrosome; cytosol; cilium

Molecular Function: protein C-terminus binding; protein domain specific binding; protein binding; protein kinase binding

Biological Process: protein localization; regulation of centriole-centriole cohesion; protein localization in organelle; organelle organization and biogenesis; mitotic cell cycle; G2/M transition of mitotic cell cycle; centriole-centriole cohesion