CECR1 sirna

CECR1 siRNA (Human)

Cat. Num. AAA8234950

• Gene Names: CECR1; PAN; ADA2; ADGF; IDGFL; SNEDS
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CECR1; CECR1 siRNA (Human); ADA2; ADGF; IDGFL; Adenosine deaminase CECR1; Cat eye syndrome critical region protein 1; CECR1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: CECR1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 511

• Applicable Applications for CECR1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human CECR1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CECR1 sirna

siRNA to inhibit CECR1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 532691754
• NCBI GeneID: 51816
• NCBI Accession #: NP_001269154.1
• NCBI GenBank Nucleotide #: NM_001282225.1
• UniProt Accession #: Q9NZK5; Q6ICF1; Q86UB6; Q8NCJ2; Q96K41; A8K9H4
• Molecular Weight: 30,668 Da
• NCBI Official Full Name: adenosine deaminase CECR1 isoform a
• NCBI Official Synonym Full Names: cat eye syndrome chromosome region, candidate 1
• NCBI Official Symbol: CECR1
• NCBI Official Synonym Symbols: PAN; ADA2; ADGF; IDGFL; SNEDS
• NCBI Protein Information: adenosine deaminase CECR1
• UniProt Protein Name: Adenosine deaminase CECR1
• Protein Family: Adenosine deaminase
• UniProt Gene Name: CECR1
• UniProt Synonym Gene Names: ADA2; ADGF; IDGFL
• UniProt Entry Name: CECR1_HUMAN

NCBI Description

This gene encodes a member of a subfamily of the adenosine deaminase protein family. The encoded protein is one of two adenosine deaminases found in humans, which regulate levels of the signaling molecule, adenosine. The encoded protein is secreted from monocytes undergoing differentiation and may regulate cell proliferation and differentiation. This gene may be responsible for some of the phenotypic features associated with cat eye syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]

Uniprot Description

CECR1: Adenosine deaminase that may contribute to the degradation of extracellular adenosine, a signaling molecule that controls a variety of cellular responses. Requires elevated adenosine levels for optimal enzyme activity. Binds to cell surfaces via proteoglycans and may play a role in the regulation of cell proliferation and differentiation, independently of its enzyme activity. Belongs to the adenosine and AMP deaminases family. ADGF subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Hydrolase; Secreted; EC 3.5.4.4; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 22q11.2

Cellular Component: extracellular space; cytosol

Molecular Function: adenosine deaminase activity; heparin binding; proteoglycan binding; protein homodimerization activity; growth factor activity; zinc ion binding; adenosine receptor binding

Biological Process: adenosine catabolic process; multicellular organismal development; hypoxanthine salvage; inosine biosynthetic process

Disease: Polyarteritis Nodosa, Childhood-onset; Sneddon Syndrome