CDK5R1 sirna

CDK5R1 siRNA (Human)

Cat. Num. AAA8232618

• Gene Names: CDK5R1; p23; p25; p35; CDK5R; NCK5A; CDK5P35; p35nck5a
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CDK5R1; CDK5R1 siRNA (Human); CDK5R; NCK5A; Cyclin-dependent kinase 5 activator 1; CDK5 activator 1; Cyclin-dependent kinase 5 regulatory subunit 1; TPKII regulatory subunit; CDK5R1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: CDK5R1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 307

• Applicable Applications for CDK5R1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human CDK5R1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CDK5R1 sirna

siRNA to inhibit CDK5R1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 4502737
• NCBI GeneID: 8851
• NCBI Accession #: NP_003876.1
• NCBI GenBank Nucleotide #: NM_003885.2
• UniProt Accession #: Q15078; Q5U0G3; E1P664
• Molecular Weight: 34,060 Da
• NCBI Official Full Name: cyclin-dependent kinase 5 activator 1
• NCBI Official Synonym Full Names: cyclin-dependent kinase 5, regulatory subunit 1 (p35)
• NCBI Official Symbol: CDK5R1
• NCBI Official Synonym Symbols: p23; p25; p35; CDK5R; NCK5A; CDK5P35; p35nck5a
• NCBI Protein Information: cyclin-dependent kinase 5 activator 1
• UniProt Protein Name: Cyclin-dependent kinase 5 activator 1
• Protein Family: Cyclin-dependent kinase 5 activator
• UniProt Gene Name: CDK5R1
• UniProt Synonym Gene Names: CDK5R; NCK5A; CDK5 activator 1; p35; p25; p23
• UniProt Entry Name: CD5R1_HUMAN

NCBI Description

The protein encoded by this gene (p35) is a neuron-specific activator of cyclin-dependent kinase 5 (CDK5); the activation of CDK5 is required for proper development of the central nervous system. The p35 form of this protein is proteolytically cleaved by calpain, generating a p25 form. The cleavage of p35 into p25 results in relocalization of the protein from the cell periphery to nuclear and perinuclear regions. P25 deregulates CDK5 activity by prolonging its activation and changing its cellular location. The p25 form accumulates in the brain neurons of patients with Alzheimer's disease. This accumulation correlates with an increase in CDK5 kinase activity, and may lead to aberrantly phosphorylated forms of the microtubule-associated protein tau, which contributes to Alzheimer's disease. [provided by RefSeq, Jul 2008]

Uniprot Description

CDK5R1: activator of cyclin-dependent kinase 5 (CDK5). Required for proper development of the central nervous system. The p35 form of this protein is cleaved by calpain, generating a p25 form, resulting in the prolonged activation and relocalization of the CDK5 from the cell periphery to nuclear and perinuclear regions. The p25 form accumulates in the brain neurons of patients with Alzheimer's disease, leading to aberrantly phosphorylated forms of the microtubule-associated protein tau, which contributes to Alzheimer's disease.

Protein type: Protein kinase, regulatory subunit

Chromosomal Location of Human Ortholog: 17q11.2

Cellular Component: intracellular membrane-bound organelle; contractile fiber; cyclin-dependent protein kinase 5 activator complex; postsynaptic density; dendrite; dendritic spine; cytosol; nucleoplasm; growth cone; cell soma; membrane; perinuclear region of cytoplasm; axon; cytoplasm; plasma membrane; nucleus; neuromuscular junction

Molecular Function: protein serine/threonine kinase activator activity; protein binding; ephrin receptor binding; cyclin-dependent protein kinase activity; cadherin binding; cyclin-dependent protein kinase 5 activator activity; kinase activity; calcium ion binding; protein kinase binding; protein kinase activity

Biological Process: superior olivary nucleus maturation; axon guidance; regulation of neuron differentiation; neuron migration; rhythmic process; negative regulation of axon extension; neuron differentiation; positive regulation of neuron apoptosis; ephrin receptor signaling pathway; cerebellum development; neuron adhesion; neurite development; layer formation in the cerebral cortex; serine phosphorylation of STAT3 protein; acetylcholine receptor signaling, muscarinic pathway; hippocampus development; peptidyl-threonine phosphorylation; axonal fasciculation; cell proliferation; embryonic development; peptidyl-serine phosphorylation; ionotropic glutamate receptor signaling pathway; regulation of actin cytoskeleton organization and biogenesis; regulation of cyclin-dependent protein kinase activity; brain development; negative regulation of transcription, DNA-dependent