CDC23 sirna

CDC23 siRNA (Human)

Cat. Num. AAA8201861

• Gene Names: CDC23; APC8; CUT23; ANAPC8
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CDC23; CDC23 siRNA (Human); ANAPC8; Cell division cycle protein 23 homolog; Anaphase-promoting complex subunit 8; APC8; Cyclosome subunit 8; CDC23 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: CDC23 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 597

• Applicable Applications for CDC23 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human CDC23 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CDC23 sirna

siRNA to inhibit CDC23 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 118402596
• NCBI GeneID: 8697
• NCBI Accession #: NP_004652.2
• NCBI GenBank Nucleotide #: NM_004661.3
• UniProt Accession #: Q9UJX2; O75433; Q53FN2; Q9BS73; A8K6E5; B4E3A2; B7WP05; D3DQB7
• Molecular Weight: 55,882 Da
• NCBI Official Full Name: cell division cycle protein 23 homolog
• NCBI Official Synonym Full Names: cell division cycle 23
• NCBI Official Symbol: CDC23
• NCBI Official Synonym Symbols: APC8; CUT23; ANAPC8
• NCBI Protein Information: cell division cycle protein 23 homolog
• UniProt Protein Name: Cell division cycle protein 23 homolog
• Protein Family: Cell division cycle protein
• UniProt Gene Name: CDC23
• UniProt Synonym Gene Names: ANAPC8; APC8
• UniProt Entry Name: CDC23_HUMAN

NCBI Description

The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae Cdc23, a protein essential for cell cycle progression through the G2/M transition. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eukaryotic cells. APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. [provided by RefSeq, Jul 2008]

Uniprot Description

CDC23: Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. Belongs to the APC8/CDC23 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation

Chromosomal Location of Human Ortholog: 5q31

Cellular Component: nucleoplasm; anaphase-promoting complex; intracellular; cytosol

Molecular Function: ubiquitin-protein ligase activity

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; ubiquitin-dependent protein catabolic process; mitosis; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; cell division; mitotic cell cycle spindle assembly checkpoint; regulation of mitotic metaphase/anaphase transition; regulation of exit from mitosis; mitotic cell cycle; mitotic metaphase plate congression; mitotic metaphase/anaphase transition