CCR1 sirna

CCR1 siRNA (Human)

Cat. Num. AAA8221234

• Gene Names: CCR1; CKR1; CD191; CKR-1; HM145; CMKBR1; MIP1aR; SCYAR1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CCR1; CCR1 siRNA (Human); CMKBR1; CMKR1; SCYAR1; C-C chemokine receptor type 1; C-C CKR-1; CC-CKR-1; CCR-1; HM145; LD78 receptor; Macrophage inflammatory protein 1-alpha receptor; MIP-1alpha-R; RANTES-R; CD antigen CD191; CCR1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: CCR1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 355

• Applicable Applications for CCR1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human CCR1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CCR1 sirna

siRNA to inhibit CCR1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 4502631
• NCBI GeneID: 1230
• NCBI Accession #: NP_001286.1
• NCBI GenBank Nucleotide #: NM_001295.2
• UniProt Accession #: P32246; Q86VA9
• Molecular Weight: 41,173 Da
• NCBI Official Full Name: C-C chemokine receptor type 1
• NCBI Official Synonym Full Names: chemokine (C-C motif) receptor 1
• NCBI Official Symbol: CCR1
• NCBI Official Synonym Symbols: CKR1; CD191; CKR-1; HM145; CMKBR1; MIP1aR; SCYAR1
• NCBI Protein Information: C-C chemokine receptor type 1
• UniProt Protein Name: C-C chemokine receptor type 1
• Protein Family: C-C chemokine receptor
• UniProt Gene Name: CCR1
• UniProt Synonym Gene Names: CMKBR1; CMKR1; SCYAR1; C-C CKR-1; CC-CKR-1; CCR-1; CCR1; MIP-1alpha-R
• UniProt Entry Name: CCR1_HUMAN

NCBI Description

This gene encodes a member of the beta chemokine receptor family, which is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. The ligands of this receptor include macrophage inflammatory protein 1 alpha (MIP-1 alpha), regulated on activation normal T expressed and secreted protein (RANTES), monocyte chemoattractant protein 3 (MCP-3), and myeloid progenitor inhibitory factor-1 (MPIF-1). Chemokines and their receptors mediated signal transduction are critical for the recruitment of effector immune cells to the site of inflammation. Knockout studies of the mouse homolog suggested the roles of this gene in host protection from inflammatory response, and susceptibility to virus and parasite. This gene and other chemokine receptor genes, including CCR2, CCRL2, CCR3, CCR5 and CCXCR1, are found to form a gene cluster on chromosome 3p. [provided by RefSeq, Jul 2008]

Uniprot Description

CCR1: Receptor for a C-C type chemokine. Binds to MIP-1-alpha, MIP-1-delta, RANTES, and MCP-3 and, less efficiently, to MIP-1- beta or MCP-1 and subsequently transduces a signal by increasing the intracellular calcium ions level. Responsible for affecting stem cell proliferation. Belongs to the G-protein coupled receptor 1 family.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR; GPCR, family 1; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 3p21

Cellular Component: integral to plasma membrane; plasma membrane; external side of plasma membrane

Molecular Function: protein binding; C-C chemokine receptor activity; chemokine receptor activity; C-C chemokine binding; phosphoinositide phospholipase C activity

Biological Process: exocytosis; metabolic process; positive regulation of osteoclast differentiation; cytokine and chemokine mediated signaling pathway; chemotaxis; cellular calcium ion homeostasis; G-protein signaling, coupled to cyclic nucleotide second messenger; negative regulation of bone mineralization; elevation of cytosolic calcium ion concentration; cell surface receptor linked signal transduction; cell-cell signaling; calcium ion transport; response to wounding; dendritic cell chemotaxis; immune response; positive regulation of calcium ion transport; inflammatory response; cell adhesion