CCNE2 sirna

CCNE2 siRNA (Human)

Cat. Num. AAA8228180

• Gene Names: CCNE2; CYCE2
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CCNE2; CCNE2 siRNA (Human); G1/S-specific cyclin-E2; CCNE2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: CCNE2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 404

• Applicable Applications for CCNE2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human CCNE2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CCNE2 sirna

siRNA to inhibit CCNE2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 17318565
• NCBI GeneID: 9134
• NCBI Accession #: NP_477097.1
• NCBI GenBank Nucleotide #: NM_057749.2
• UniProt Accession #: O96020; O95439
• Molecular Weight: 41,465 Da
• NCBI Official Full Name: G1/S-specific cyclin-E2
• NCBI Official Synonym Full Names: cyclin E2
• NCBI Official Symbol: CCNE2
• NCBI Official Synonym Symbols: CYCE2
• NCBI Protein Information: G1/S-specific cyclin-E2
• UniProt Protein Name: G1/S-specific cyclin-E2
• Protein Family: G1/S-specific cyclin
• UniProt Gene Name: CCNE2
• UniProt Entry Name: CCNE2_HUMAN

NCBI Description

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2. This cyclin has been shown to specifically interact with CIP/KIP family of CDK inhibitors, and plays a role in cell cycle G1/S transition. The expression of this gene peaks at the G1-S phase and exhibits a pattern of tissue specificity distinct from that of cyclin E1. A significantly increased expression level of this gene was observed in tumor-derived cells. [provided by RefSeq, Jul 2008]

Uniprot Description

CCNE2: Essential for the control of the cell cycle at the late G1 and early S phase. Interacts with the CDK2 (in vivo) and CDK3 (in vitro) protein kinases to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex. Activated by papilloma viral oncoproteins E6 and E7 which bind to and inactivate p53 and Rb, respectively. According to PubMed:9858585, highest levels of expression in adult testis, thymus and brain. Lower levels in placenta, spleen and colon. Consistently elevated levels in tumor- derived cells compared to non-transformed proliferating cells. According to PubMed:9840927: low levels in thymus, prostate, brain, skeletal muscle, and kidney. Elevated levels in lung. According to PubMed:9840943 highly expressed in testis, placenta, thymus and brain. In a lesser extent in small intestine and colon. Belongs to the cyclin family. Cyclin E subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Activator; Cell cycle regulation

Chromosomal Location of Human Ortholog: 8q22.1

Cellular Component: nucleoplasm; cytosol

Molecular Function: protein binding; cyclin-dependent protein kinase regulator activity

Biological Process: DNA replication initiation; cell division; mitotic cell cycle; regulation of cyclin-dependent protein kinase activity; cell cycle checkpoint; G1/S transition of mitotic cell cycle