CCNC sirna

CCNC siRNA (Human)

Cat. Num. AAA8220130

• Gene Names: CCNC; CycC
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CCNC; CCNC siRNA (Human); Cyclin-C; SRB11 homolog; hSRB11; CCNC sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: CCNC siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 198

• Applicable Applications for CCNC sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human CCNC gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CCNC sirna

siRNA to inhibit CCNC expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 61676093
• NCBI GeneID: 892
• NCBI Accession #: NP_001013417.1
• NCBI GenBank Nucleotide #: NM_001013399.1
• UniProt Accession #: P24863; Q9H543; B4DPZ1
• Molecular Weight: 22,945 Da
• NCBI Official Full Name: cyclin-C isoform b
• NCBI Official Synonym Full Names: cyclin C
• NCBI Official Symbol: CCNC
• NCBI Official Synonym Symbols: CycC
• NCBI Protein Information: cyclin-C
• UniProt Protein Name: Cyclin-C
• UniProt Gene Name: CCNC
• UniProt Synonym Gene Names: hSRB11
• UniProt Entry Name: CCNC_HUMAN

NCBI Description

The protein encoded by this gene is a member of the cyclin family of proteins. The encoded protein interacts with cyclin-dependent kinase 8 and induces the phophorylation of the carboxy-terminal domain of the large subunit of RNA polymerase II. The level of mRNAs for this gene peaks in the G1 phase of the cell cycle. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

CCNC: Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Binds to and activates cyclin-dependent kinase CDK8 that phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex. Belongs to the cyclin family. Cyclin C subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation

Chromosomal Location of Human Ortholog: 6q21

Cellular Component: nucleoplasm; DNA-directed RNA polymerase II, holoenzyme; Srb-mediator complex

Molecular Function: protein binding; protein kinase binding

Biological Process: transcription initiation from RNA polymerase II promoter; Notch signaling pathway; transcription, DNA-dependent; transforming growth factor beta receptor signaling pathway; gene expression; positive regulation of transcription from RNA polymerase II promoter; regulation of cyclin-dependent protein kinase activity