CCNB3 sirna

CCNB3 siRNA (Human)

Cat. Num. AAA8240243

• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CCNB3; CCNB3 siRNA (Human); CYCB3; G2/mitotic-specific cyclin-B3; CCNB3 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: CCNB3 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1395

• Applicable Applications for CCNB3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human CCNB3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CCNB3 sirna

siRNA to inhibit CCNB3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 90669307
• NCBI GeneID: 85417
• NCBI Accession #: NP_149020.2
• NCBI GenBank Nucleotide #: NM_033031.2
• UniProt Accession #: Q8WWL7; Q96SB5; Q96SB6; Q96SB7; Q9NT38; B1AQI5; B1AQI6
• Molecular Weight: 12,195 Da
• NCBI Official Full Name: G2/mitotic-specific cyclin-B3 isoform 3
• NCBI Official Synonym Full Names: cyclin B3
• NCBI Official Symbol: CCNB3
• NCBI Protein Information: G2/mitotic-specific cyclin-B3
• UniProt Protein Name: G2/mitotic-specific cyclin-B3
• UniProt Gene Name: CCNB3
• UniProt Synonym Gene Names: CYCB3
• UniProt Entry Name: CCNB3_HUMAN

NCBI Description

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as positive regulators of cyclin-dependent kinases (CDKs), and thereby play an essential role in the control of the cell cycle. Different cyclins exhibit distinct expression and degradation patterns, which contribute to the temporal coordination of each mitotic event. Studies of similar genes in chicken and drosophila suggest that this cyclin may associate with CDC2 and CDK2 kinases, and may be required for proper spindle reorganization and restoration of the interphase nucleus. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Oct 2011]

Uniprot Description

CCNB3: Cyclins are positive regulatory subunits of the cyclin- dependent kinases (CDKs), and thereby play an essential role in the control of the cell cycle, notably via their destruction during cell division. Its tissue specificity suggest that it may be required during early meiotic prophase I. Belongs to the cyclin family. Cyclin AB subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation

Chromosomal Location of Human Ortholog: Xp11

Cellular Component: nucleoplasm

Molecular Function: protein binding; protein kinase binding

Biological Process: meiotic cell cycle; cell division; regulation of G2/M transition of mitotic cell cycle; regulation of cyclin-dependent protein kinase activity