CASP12 sirna

CASP12 siRNA (Human)

Cat. Num. AAA8211681

• Gene Names: CASP12; CASP-12; CASP12P1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CASP12; CASP12 siRNA (Human); Inactive caspase-12; CASP-12; CASP12 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: CASP12 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 341

• Applicable Applications for CASP12 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human CASP12 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CASP12 sirna

siRNA to inhibit CASP12 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 617418490
• NCBI GeneID: 100506742
• NCBI Accession #: NP_001177945.2
• NCBI GenBank Nucleotide #: NM_001191016.2
• UniProt Accession #: Q6UXS9; D6RBN7
• Molecular Weight: 29,399 Da
• NCBI Official Full Name: inactive caspase-12
• NCBI Official Synonym Full Names: caspase 12 (gene/pseudogene)
• NCBI Official Symbol: CASP12
• NCBI Official Synonym Symbols: CASP-12; CASP12P1
• NCBI Protein Information: inactive caspase-12
• UniProt Protein Name: Inactive caspase-12
• Protein Family: Inactive caspase
• UniProt Gene Name: CASP12
• UniProt Synonym Gene Names: CASP-12
• UniProt Entry Name: CASPC_HUMAN

NCBI Description

Caspases are cysteine proteases that cleave C-terminal aspartic acid residues on their substrate molecules. This gene is most highly related to members of the ICE subfamily of caspases that process inflammatory cytokines. In rodents, the homolog of this gene mediates apoptosis in response to endoplasmic reticulum stress. However, in humans this gene contains a polymorphism for the presence or absence of a premature stop codon. The majority of human individuals have the premature stop codon and produce a truncated non-functional protein. The read-through codon occurs primarily in individuals of African descent and carriers have endotoxin hypo-responsiveness and an increased susceptibility to severe sepsis. Several alternatively spliced transcript variants have been noted for this gene. [provided by RefSeq, Feb 2011]

Uniprot Description

CASP12: Has no protease activity. May reduce cytokine release in response to bacterial lipopolysaccharide during infections. Reduces activation of NF-kappa-B in response to TNF. Detected in heart, kidney, liver, lung, pancreas, small intestine, spleen, stomach, thymus and testis. Belongs to the peptidase C14A family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.22.-; Protease

Chromosomal Location of Human Ortholog: 11q22.3

Cellular Component: cytoplasm

Molecular Function: cysteine-type endopeptidase activity

Biological Process: regulation of apoptosis; unfolded protein response; regulation of inflammatory response; proteolysis