CARD16 sirna

CARD16 siRNA (Human)

Cat. Num. AAA8221007

• Gene Names: CARD16; COP; COP1; PSEUDO-ICE
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CARD16; CARD16 siRNA (Human); COP; COP1; Caspase recruitment domain-containing protein 16; CARD only domain-containing protein 1; Caspase-1 inhibitor COP; Pseudo interleukin-1 beta converting enzyme; Pseudo-ICE; CARD16 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: CARD16 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 97

• Applicable Applications for CARD16 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human CARD16 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CARD16 sirna

siRNA to inhibit CARD16 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 62953113
• NCBI GeneID: 114769
• NCBI Accession #: NP_001017534.1
• NCBI GenBank Nucleotide #: NM_001017534.1
• UniProt Accession #: Q5EG05; Q96RJ9
• Molecular Weight: 10,737 Da
• NCBI Official Full Name: caspase recruitment domain-containing protein 16 isoform 1
• NCBI Official Synonym Full Names: caspase recruitment domain family, member 16
• NCBI Official Symbol: CARD16
• NCBI Official Synonym Symbols: COP; COP1; PSEUDO-ICE
• NCBI Protein Information: caspase recruitment domain-containing protein 16
• UniProt Protein Name: Caspase recruitment domain-containing protein 16
• Protein Family: Caspase recruitment domain-containing protein
• UniProt Gene Name: CARD16
• UniProt Synonym Gene Names: COP; COP1; CARD-only protein 1; Pseudo-ICE; Pseudo-IL1B-converting enzyme
• UniProt Entry Name: CAR16_HUMAN

Uniprot Description

CARD16: Caspase inhibitor. Acts as a regulator of procaspase- 1/CASP1 activation implicated in the regulation of the proteolytic maturation of pro-interleukin-1 beta (IL1B) and its release during inflammation. Inhibits the release of IL1B in response to LPS in monocytes. Also induces NF-kappa-B activation during the pro- inflammatory cytokine response. Also able to inhibit CASP1- mediated neuronal cell death, TNF-alpha, hypoxia-, UV-, and staurosporine-mediated cell death but not ER stress-mediated cell death. Acts by preventing activation of caspases CASP1 and CASP4, possibly by preventing the interaction between CASP1 and RIPK2. Down-regulated in patients suffering of Huntington disease. Homooligomer. Interacts with CASP1, CASP4, CARD8 and RIPK2. 2 isoforms of the human protein are produced by alternative splicing

Chromosomal Location of Human Ortholog: 11q22

Molecular Function: cysteine protease inhibitor activity

Biological Process: regulation of apoptosis