CARD14 sirna

CARD14 siRNA (Human)

Cat. Num. AAA8228860

• Gene Names: CARD14; PRP; PSS1; BIMP2; CARMA2; PSORS2
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CARD14; CARD14 siRNA (Human); CARMA2; Caspase recruitment domain-containing protein 14; CARD-containing MAGUK protein 2; Carma 2; CARD14 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: CARD14 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 740

• Applicable Applications for CARD14 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human CARD14 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CARD14 sirna

siRNA to inhibit CARD14 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 384229040
• NCBI GeneID: 79092
• NCBI Accession #: NP_001244899.1
• NCBI GenBank Nucleotide #: NM_001257970.1
• UniProt Accession #: Q9BXL6; Q9BVB5; B8QQJ3
• Molecular Weight: 48,505 Da
• NCBI Official Full Name: caspase recruitment domain-containing protein 14 isoform 3
• NCBI Official Synonym Full Names: caspase recruitment domain family, member 14
• NCBI Official Symbol: CARD14
• NCBI Official Synonym Symbols: PRP; PSS1; BIMP2; CARMA2; PSORS2
• NCBI Protein Information: caspase recruitment domain-containing protein 14
• UniProt Protein Name: Caspase recruitment domain-containing protein 14
• Protein Family: Caspase recruitment domain-containing protein
• UniProt Gene Name: CARD14
• UniProt Synonym Gene Names: CARMA2; Carma 2
• UniProt Entry Name: CAR14_HUMAN

NCBI Description

This gene encodes a caspase recruitment domain-containing protein that is a member of the membrane-associated guanylate kinase (MAGUK) family of proteins. Members of this protein family are scaffold proteins that are involved in a diverse array of cellular processes including cellular adhesion, signal transduction and cell polarity control. This protein has been shown to specifically interact with BCL10, a protein known to function as a positive regulator of cell apoptosis and NF-kappaB activation. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Apr 2012]

Uniprot Description

CARD14: a protein containing a caspase recruitment domain (CARD) that functions as an activator of BCL10 and NF-kappaB signaling. CARD is a protein module that consists of 6 or 7 antiparallel alpha helices. It participates in signaling through highly specific protein-protein homophilic interactions. CARD proteins function as scaffolds for the assembly of multiprotein complexes at specialized regions of the plasma membrane. The CARD domain of CARD14 associates specifically with the CARD domains of CARD10 and BCL10, a signaling protein that activates NF-kB through the IkappaB kinase complex in response to upstream stimuli. When expressed in cells, CARD14 activates NF-kappaB and induces the phosphorylation of BCL10. A subgroup of primary lymphomas of the central nervous system have higher levels of CARD14 expression.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 17q25

Cellular Component: cytoplasm; plasma membrane

Molecular Function: CARD domain binding

Biological Process: tumor necrosis factor-mediated signaling pathway; apoptosis; activation of NF-kappaB-inducing kinase; positive regulation of protein amino acid phosphorylation; activation of NF-kappaB transcription factor; negative regulation of apoptosis

Disease: Psoriasis 2; Pityriasis Rubra Pilaris