CAMK4 sirna

CAMK4 siRNA (Human)

Cat. Num. AAA8238392

• Gene Names: CAMK4; IV; caMK; CaMK IV; CaMK-GR
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CAMK4; CAMK4 siRNA (Human); CAMK; CAMK-GR; CAMKIV; Calcium/calmodulin-dependent protein kinase type IV; CaMK IV; CaM kinase-GR; CAMK4 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: CAMK4 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 473

• Applicable Applications for CAMK4 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human CAMK4 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CAMK4 sirna

siRNA to inhibit CAMK4 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 4502557
• NCBI GeneID: 814
• NCBI Accession #: NP_001735.1
• NCBI GenBank Nucleotide #: NM_001744.4
• UniProt Accession #: Q16566; D3DSZ7
• Molecular Weight: 51,926 Da
• NCBI Official Full Name: calcium/calmodulin-dependent protein kinase type IV
• NCBI Official Synonym Full Names: calcium/calmodulin-dependent protein kinase IV
• NCBI Official Symbol: CAMK4
• NCBI Official Synonym Symbols: IV; caMK; CaMK IV; CaMK-GR
• NCBI Protein Information: calcium/calmodulin-dependent protein kinase type IV
• UniProt Protein Name: Calcium/calmodulin-dependent protein kinase type IV
• Protein Family: Calcium/calmodulin-dependent protein kinase
• UniProt Gene Name: CAMK4
• UniProt Synonym Gene Names: CAMK; CAMK-GR; CAMKIV; CaMK IV
• UniProt Entry Name: KCC4_HUMAN

NCBI Description

The product of this gene belongs to the serine/threonine protein kinase family, and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. This enzyme is a multifunctional serine/threonine protein kinase with limited tissue distribution, that has been implicated in transcriptional regulation in lymphocytes, neurons and male germ cells. [provided by RefSeq, Jul 2008]

Uniprot Description

CAMK4: a protein kinase of the CAMK1 family. Implicated in transcriptional regulation in lymphocytes, neurons and male germ cells. Two alternatively sliced isoforms have been described.

Protein type: EC 2.7.11.17; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Protein kinase, CAMK; CAMK group; CAMK1 family

Chromosomal Location of Human Ortholog: 5q21.3

Cellular Component: nucleoplasm; nucleolus; cytosol

Molecular Function: calmodulin binding; calmodulin-dependent protein kinase activity; ATP binding

Biological Process: epidermal growth factor receptor signaling pathway; mitochondrion organization and biogenesis; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; regulation of T cell differentiation in the thymus; positive regulation of transcription, DNA-dependent; organelle organization and biogenesis; myeloid dendritic cell differentiation; nucleocytoplasmic transport; signal transduction; protein amino acid phosphorylation; positive regulation of protein export from nucleus; synaptic transmission; regulation of osteoclast differentiation; long-term memory; phospholipase C activation; innate immune response; nerve-nerve synaptic transmission; inflammatory response; myeloid dendritic cell cytokine production