CAMK2N2 sirna

CAMK2N2 siRNA (Human)

Cat. Num. AAA8218460

• Gene Names: CAMK2N2; CAMKIIN; CAM-KIIN
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CAMK2N2; CAMK2N2 siRNA (Human); Calcium/calmodulin-dependent protein kinase II inhibitor 2; CaM-KII inhibitory protein; CaM-KIIN; CAMK2N2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: CAMK2N2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 79

• Applicable Applications for CAMK2N2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human CAMK2N2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CAMK2N2 sirna

siRNA to inhibit CAMK2N2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 15147234
• NCBI GeneID: 94032
• NCBI Accession #: NP_150284.1
• NCBI GenBank Nucleotide #: NM_033259.2
• UniProt Accession #: Q96S95
• Molecular Weight: 8,658 Da
• NCBI Official Full Name: calcium/calmodulin-dependent protein kinase II inhibitor 2
• NCBI Official Synonym Full Names: calcium/calmodulin-dependent protein kinase II inhibitor 2
• NCBI Official Symbol: CAMK2N2
• NCBI Official Synonym Symbols: CAMKIIN; CAM-KIIN
• NCBI Protein Information: calcium/calmodulin-dependent protein kinase II inhibitor 2
• UniProt Protein Name: Calcium/calmodulin-dependent protein kinase II inhibitor 2
• Protein Family: Calcium/calmodulin-dependent protein kinase II inhibitor
• UniProt Gene Name: CAMK2N2
• UniProt Synonym Gene Names: CaM-KIIN
• UniProt Entry Name: CK2N2_HUMAN

NCBI Description

This gene encodes a protein that is highly similar to the rat CaM-KII inhibitory protein, an inhibitor of calcium/calmodulin-dependent protein kinase II (CAMKII). CAMKII regulates numerous physiological functions, including neuronal synaptic plasticity through the phosphorylation of alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid-type glutamate (AMPA) receptors. Studies of the similar protein in rat suggest that this protein may function as a negative regulator of CaM-KII and may act to inhibit the phosphorylation of AMPA receptors. [provided by RefSeq, Jul 2008]

Uniprot Description

CAMK2N2: Potent and specific cellular inhibitor of CaM-kinase II (CAMK2). Traps Ca(2+)/calmodulin on CAMK2. May play an important role in the regulation of cell growth when overexpressed in colon adenocarcinoma LoVo cells. Traps Ca(2+)/calmodulin on CAMK2. Belongs to the CAMK2N family.

Protein type: Protein kinase, regulatory subunit; Inhibitor

Chromosomal Location of Human Ortholog: 3q27.1

Cellular Component: centrosome; cytosol; nucleus

Molecular Function: calcium-dependent protein kinase inhibitor activity; protein kinase binding

Biological Process: negative regulation of protein kinase activity