CAMK2A sirna

CAMK2A siRNA (Human)

Cat. Num. AAA8240417

• Gene Names: CAMK2A; CAMKA
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CAMK2A; CAMK2A siRNA (Human); CAMKA; KIAA0968; Calcium/calmodulin-dependent protein kinase type II subunit alpha; CaM kinase II subunit alpha; CaMK-II subunit alpha; CAMK2A sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: CAMK2A siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 478

• Applicable Applications for CAMK2A sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human CAMK2A gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CAMK2A sirna

siRNA to inhibit CAMK2A expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 25952118
• NCBI GeneID: 815
• NCBI Accession #: NP_741960.1
• NCBI GenBank Nucleotide #: NM_171825.2
• UniProt Accession #: Q9UQM7; Q9UL21; Q9Y2H4; Q9Y352
• Molecular Weight: 55,320 Da
• NCBI Official Full Name: calcium/calmodulin-dependent protein kinase type II subunit alpha isoform 2
• NCBI Official Synonym Full Names: calcium/calmodulin-dependent protein kinase II alpha
• NCBI Official Symbol: CAMK2A
• NCBI Official Synonym Symbols: CAMKA
• NCBI Protein Information: calcium/calmodulin-dependent protein kinase type II subunit alpha
• UniProt Protein Name: Calcium/calmodulin-dependent protein kinase type II subunit alpha
• Protein Family: Calcium/calmodulin-dependent protein kinase
• UniProt Gene Name: CAMK2A
• UniProt Synonym Gene Names: CAMKA; KIAA0968; CaM kinase II subunit alpha; CaMK-II subunit alpha
• UniProt Entry Name: KCC2A_HUMAN

NCBI Description

The product of this gene belongs to the serine/threonine protein kinases family, and to the Ca(2+)/calmodulin-dependent protein kinases subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. This calcium calmodulin-dependent protein kinase is composed of four different chains: alpha, beta, gamma, and delta. The alpha chain encoded by this gene is required for hippocampal long-term potentiation (LTP) and spatial learning. In addition to its calcium-calmodulin (CaM)-dependent activity, this protein can undergo autophosphorylation, resulting in CaM-independent activity. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Nov 2008]

Uniprot Description

CAMK2A: a protein kinase of the CAMK2 family. A prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses that may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. The holoenzyme is composed of four different chains: alpha, beta, gamma, and delta. The different chains assemble into homo- or heteromultimeric holoenzymes composed of 8 to 12 subunits. May interact with BAALC, MPDZ, SYN1 and synGAP. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.7.11.17; Protein kinase, CAMK; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); CAMK group; CAMK2 family

Chromosomal Location of Human Ortholog: 5q32

Cellular Component: presynaptic membrane; nucleoplasm; mitochondrion; plasma membrane; cell junction; nucleus; cytosol

Molecular Function: calmodulin binding; protein serine/threonine kinase activity; glutamate receptor binding; protein binding; protein homodimerization activity; calmodulin-dependent protein kinase activity; kinase activity; ATP binding

Biological Process: synaptic transmission; peptidyl-serine phosphorylation; calcium ion transport; cytokine and chemokine mediated signaling pathway; protein amino acid autophosphorylation; regulation of neurotransmitter secretion; positive regulation of calcium ion transport; protein amino acid phosphorylation; activation of NF-kappaB transcription factor; G1/S transition of mitotic cell cycle; regulation of neuronal synaptic plasticity