C1QBP sirna

C1QBP siRNA (Human)

Cat. Num. AAA8214064

• Gene Names: C1QBP; p32; HABP1; gC1qR; GC1QBP; SF2p32; gC1Q-R
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: C1QBP; C1QBP siRNA (Human); GC1QBP; HABP1; SF2P32; Complement component 1 Q subcomponent-binding protein mitochondrial; ASF/SF2-associated protein p32; Glycoprotein gC1qBP; C1qBP; Hyaluronan-binding protein 1; Mitochondrial matrix protein p32; gC1q-R protein; p33; C1QBP sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: C1QBP siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 282

• Applicable Applications for C1QBP sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human C1QBP gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for C1QBP sirna

siRNA to inhibit C1QBP expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 4502491
• NCBI GeneID: 708
• NCBI Accession #: NP_001203.1
• NCBI GenBank Nucleotide #: NM_001212.3
• UniProt Accession #: Q07021; Q2HXR8; Q9NNY8
• Molecular Weight: 31,362 Da
• NCBI Official Full Name: complement component 1 Q subcomponent-binding protein, mitochondrial
• NCBI Official Synonym Full Names: complement component 1, q subcomponent binding protein
• NCBI Official Symbol: C1QBP
• NCBI Official Synonym Symbols: p32; HABP1; gC1qR; GC1QBP; SF2p32; gC1Q-R
• NCBI Protein Information: complement component 1 Q subcomponent-binding protein, mitochondrial
• UniProt Protein Name: Complement component 1 Q subcomponent-binding protein, mitochondrial
• Protein Family: Complement component 1 Q subcomponent-binding protein
• UniProt Gene Name: C1QBP
• UniProt Synonym Gene Names: GC1QBP; HABP1; SF2P32; C1qBP
• UniProt Entry Name: C1QBP_HUMAN

NCBI Description

The human complement subcomponent C1q associates with C1r and C1s in order to yield the first component of the serum complement system. The protein encoded by this gene is known to bind to the globular heads of C1q molecules and inhibit C1 activation. This protein has also been identified as the p32 subunit of pre-mRNA splicing factor SF2, as well as a hyaluronic acid-binding protein. [provided by RefSeq, Jul 2008]

Uniprot Description

C1QBP: a multifunctional and multicompartmental protein involved in inflammation and infection processes, ribosome biogenesis, regulation of apoptosis, transcriptional regulation and pre-mRNA splicing. Originally identified via its binding interactions with Splicing Factor (SF-2). Multiple, diverse binding partners of C1QBP were subsequently identified, including the globular heads of complement component C1q, hyaluronic acid, selected protein kinases, the tumor suppressor ARF, and multiple antigens of bacterial and viral origin. Overexpressed in a number of cancer cell types, and has been implicated in the Warburg effect, whereby cancer cells shift their metabolism from oxidative phosphorylation to glycolysis. Inhibits the Mitochondrial Permeability Transition (MPT) pore, possibly serving a protective function against damage from oxidative stress. Binding to C1q inhibits C1. In complex with cytokeratin-1/KRT1 is a high affinty receptor for kininogen-1/HMWK. The secreted form may enhance both extrinsic and intrinsic coagulation pathways. The cell surface form may require docking with transmembrane proteins for downstream signaling which might be specific for a cell-type or response. Belongs to the MAM33 family.

Protein type: Nucleolus; Motility/polarity/chemotaxis; Mitochondrial; Receptor, misc.

Chromosomal Location of Human Ortholog: 17p13.3

Cellular Component: extracellular space; cell surface; mitochondrion; membrane; mitochondrial matrix; cytoplasm; plasma membrane; nucleolus; cytosol; nucleus

Molecular Function: mRNA binding; protein binding; adrenergic receptor binding; protein kinase C binding; complement component C1q binding; hyaluronic acid binding; kininogen binding; transcription factor binding; transcription corepressor activity

Biological Process: phosphoinositide 3-kinase cascade; negative regulation of interleukin-12 production; positive regulation of cell adhesion; viral reproduction; transcription, DNA-dependent; apoptosis; positive regulation of apoptosis; RNA splicing; negative regulation of defense response to virus; negative regulation of transcription from RNA polymerase II promoter; positive regulation of protein kinase B signaling cascade; negative regulation of nuclear mRNA splicing, via spliceosome; negative regulation of interferon-gamma production; regulation of complement activation; innate immune response; immune response; mRNA processing; blood coagulation; complement activation, classical pathway; blood coagulation, intrinsic pathway; mature ribosome assembly