BNC1 sirna

BNC1 siRNA (Human)

Cat. Num. AAA8215016

• Gene Names: BNC1; BNC; BSN1; HsT19447
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: BNC1; BNC1 siRNA (Human); BNC; Zinc finger protein basonuclin-1; BNC1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: BNC1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 987

• Applicable Applications for BNC1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human BNC1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for BNC1 sirna

siRNA to inhibit BNC1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 667714407
• NCBI GeneID: 646
• NCBI Accession #: NP_001288135.1
• NCBI GenBank Nucleotide #: NM_001301206.1
• UniProt Accession #: Q01954; Q15840
• Molecular Weight: 110,972 Da
• NCBI Official Full Name: zinc finger protein basonuclin-1 isoform b
• NCBI Official Synonym Full Names: basonuclin 1
• NCBI Official Symbol: BNC1
• NCBI Official Synonym Symbols: BNC; BSN1; HsT19447
• NCBI Protein Information: zinc finger protein basonuclin-1
• UniProt Protein Name: Zinc finger protein basonuclin-1
• Protein Family: Cruciferin
• UniProt Gene Name: BNC1
• UniProt Synonym Gene Names: BNC
• UniProt Entry Name: BNC1_HUMAN

NCBI Description

The protein encoded by this gene is a zinc finger protein present in the basal cell layer of the epidermis and in hair follicles. It is also found in abundance in the germ cells of testis and ovary. This protein is thought to play a regulatory role in keratinocyte proliferation and it may also be a regulator for rRNA transcription. Alternative splicing of this gene results in multiple transcript variants, and multiple polyadenylation sites are indicated.[provided by RefSeq, Jul 2014]

Uniprot Description

basonuclin 1: Likely to be a transcription factor specific for squamous epithelium and for the constituent keratinocytes at a stage either prior to or at the very beginning of terminal differentiation. May play a role in the differentiation of spermatozoa and oocytes.

Protein type: Transcription factor; C2H2-type zinc finger protein

Chromosomal Location of Human Ortholog: 15q25.2

Cellular Component: cytoplasm; nucleus

Molecular Function: DNA binding; metal ion binding; transcription factor activity

Biological Process: regulation of transcription from RNA polymerase II promoter; regulation of transcription from RNA polymerase I promoter; epidermis development; wound healing; transcription, DNA-dependent; positive regulation of cell proliferation; chromosome organization and biogenesis; positive regulation of epithelial cell proliferation