BMI1 sirna

BMI1 siRNA (Human)

Cat. Num. AAA8204877

• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: BMI1; BMI1 siRNA (Human); PCGF4; RNF51; Polycomb complex protein BMI-1; Polycomb group RING finger protein 4; RING finger protein 51; BMI1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: BMI1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 469

• Applicable Applications for BMI1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human BMI1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for BMI1 sirna

siRNA to inhibit BMI1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 323276616
• NCBI GeneID: 100532731
• NCBI Accession #: NP_001190991.1
• NCBI GenBank Nucleotide #: NM_001204062.1
• UniProt Accession #: P35226; Q16030; Q5T8Z3; Q96F37
• Molecular Weight: 36,949 Da
• NCBI Official Full Name: COMMD3-BMI1 read-through protein
• NCBI Official Synonym Full Names: COMMD3-BMI1 readthrough
• NCBI Official Symbol: COMMD3-BMI1
• NCBI Protein Information: COMMD3-BMI1 read-through protein
• UniProt Protein Name: Polycomb complex protein BMI-1
• Protein Family: Polycomb complex protein
• UniProt Gene Name: BMI1
• UniProt Synonym Gene Names: PCGF4; RNF51
• UniProt Entry Name: BMI1_HUMAN

NCBI Description

This locus represents naturally occurring read-through transcription between the neighboring COMM domain-containing protein 3 and polycomb complex protein BMI-1 genes on chromosome 10. The read-through transcript produces a fusion protein that shares sequence identity with each individual gene product. [provided by RefSeq, Feb 2011]

Uniprot Description

BMI1: Component of a Polycomb group (PcG) multiprotein PRC1- like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2. Component of a PRC1-like complex. Interacts with RING1 and RING2. Interacts vwith CBX7 and CBX8. Interacts with SPOP. Part of a complex consisting of BMI1, CUL3 and SPOP. Interacts with E4F1.

Protein type: Ligase; Ubiquitin ligase; Motility/polarity/chemotaxis; Oncoprotein; EC 6.3.2.-; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 10p11.23

Cellular Component: nucleoplasm; nuclear body; heterochromatin; cytoplasm; PcG protein complex; nucleus; ubiquitin ligase complex

Molecular Function: protein binding; zinc ion binding; sequence-specific DNA binding; ubiquitin-protein ligase activity; chromatin binding

Biological Process: transcription, DNA-dependent; in utero embryonic development; positive regulation of immature T cell proliferation in the thymus; negative regulation of transcription from RNA polymerase II promoter; humoral immune response; embryonic skeletal morphogenesis; segment specification; rostrocaudal neural tube patterning; positive regulation of fibroblast proliferation; regulation of gene expression; positive regulation of ubiquitin-protein ligase activity; histone ubiquitination; positive regulation of B cell proliferation; DNA methylation; somatic stem cell division; hemopoiesis; brain development; histone acetylation