BFSP1 sirna

BFSP1 siRNA (Human)

Cat. Num. AAA8200586

• Gene Names: BFSP1; CP94; CP115; LIFL-H; CTRCT33
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: BFSP1; BFSP1 siRNA (Human); Filensin; Beaded filament structural protein 1; Lens fiber cell beaded-filament structural protein CP 115; CP115; Lens intermediate filament-like heavy; LIFL-H; BFSP1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: BFSP1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 540

• Applicable Applications for BFSP1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human BFSP1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for BFSP1 sirna

siRNA to inhibit BFSP1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 239787151
• NCBI GeneID: 631
• NCBI Accession #: NP_001155177.1
• NCBI GenBank Nucleotide #: NM_001161705.1
• UniProt Accession #: Q12934; O43595; O76034; O95676; Q8IVZ6; Q9HBX4; F5H0G1
• Molecular Weight: 58,569 Da
• NCBI Official Full Name: filensin isoform 2
• NCBI Official Synonym Full Names: beaded filament structural protein 1, filensin
• NCBI Official Symbol: BFSP1
• NCBI Official Synonym Symbols: CP94; CP115; LIFL-H; CTRCT33
• NCBI Protein Information: filensin
• UniProt Protein Name: Filensin
• Protein Family: Filensin
• UniProt Gene Name: BFSP1
• UniProt Synonym Gene Names: CP115; LIFL-H
• UniProt Entry Name: BFSP1_HUMAN

NCBI Description

This gene encodes a lens-specific intermediate filament-like protein named filensin. The encoded protein is expressed in lens fiber cells after differentiation has begun. This protein functions as a component of the beaded filament which is a cytoskeletal structure found in lens fiber cells. Mutations in this gene are the cause of autosomal recessive cortical juvenile-onset cataract. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Uniprot Description

BFSP1: Defects in BFSP1 are the cause of cataract cortical juvenile-onset (CCJO). A juvenile-onset cataract with opacities restricted to the cortex of the lens, not involving the nucleus. Belongs to the intermediate filament family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 20p12.1

Cellular Component: mitochondrion; cytoplasm; plasma membrane; intermediate filament; actin cytoskeleton

Molecular Function: structural constituent of cytoskeleton; structural constituent of eye lens

Biological Process: cell maturation

Disease: Cataract 33