BCR sirna

BCR siRNA (Human)

Cat. Num. AAA8239913

• Gene Names: BCR; ALL; CML; PHL; BCR1; D22S11; D22S662
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: BCR; BCR siRNA (Human); BCR1; D22S11; Breakpoint cluster region protein; Renal carcinoma antigen NY-REN-26; BCR sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: BCR siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1271

• Applicable Applications for BCR sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human BCR gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for BCR sirna

siRNA to inhibit BCR expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 82546843
• NCBI GeneID: 613
• NCBI Accession #: NP_004318.3
• NCBI GenBank Nucleotide #: NM_004327.3
• UniProt Accession #: P11274; P78501; Q12842; Q4LE80; Q6NZI3
• Molecular Weight: 137,729 Da
• NCBI Official Full Name: breakpoint cluster region protein isoform 1
• NCBI Official Synonym Full Names: breakpoint cluster region
• NCBI Official Symbol: BCR
• NCBI Official Synonym Symbols: ALL; CML; PHL; BCR1; D22S11; D22S662
• NCBI Protein Information: breakpoint cluster region protein
• UniProt Protein Name: Breakpoint cluster region protein
• Protein Family: Bicyclomycin resistance protein
• UniProt Gene Name: BCR
• UniProt Synonym Gene Names: BCR1; D22S11
• UniProt Entry Name: BCR_HUMAN

NCBI Description

A reciprocal translocation between chromosomes 22 and 9 produces the Philadelphia chromosome, which is often found in patients with chronic myelogenous leukemia. The chromosome 22 breakpoint for this translocation is located within the BCR gene. The translocation produces a fusion protein which is encoded by sequence from both BCR and ABL, the gene at the chromosome 9 breakpoint. Although the BCR-ABL fusion protein has been extensively studied, the function of the normal BCR gene product is not clear. The protein has serine/threonine kinase activity and is a GTPase-activating protein for p21rac. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Bcr: a protein with serine/threonine protein kinase activity and is a GTPase-activating protein (GAP) for RAC1 and CDC42. The amino-terminal region of Bcr contains an oligomerization domain, a serine/threonine kinase domain and a region that binds SH2 domains. The middle of the protein has a PH domain and a region of sequence similarity to the guanine nucleotide exchange factors for the Rho family of GTP binding proteins. The carboxy-terminal region promotes the exchange of RAC or CDC42-bound GDP by GTP, thereby activating them. A breakpoint cluster region protein that participates in a t(9;22)(q34;q11) chromosomal translocation that produces a BCR-ABL oncogene responsible for chronic myeloid leukemia (CML), acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL). The function of wild type Bcr in cells remains unclear. PDGF receptor may use Bcr as a downstream signaling mediator. Tyr177 of human Bcr, phosphorylated in the Bcr-Abl fusion protein, provides a docking site for Gab2 and GRB2, and is important in the transforming activity of Bcr-Abl. Sequence variants of the Bcr protein may be associated with bipolar disorder. Two alternatively spliced isoforms of the human protein have been reported.

Protein type: EC 2.7.11.1; GEFs, Rac/Rho; Protein kinase, atypical; Protein kinase, Ser/Thr (non-receptor); GAPs, Rac/Rho; Kinase, protein; Oncoprotein; GAPs; ATYPICAL group; BCR family

Chromosomal Location of Human Ortholog: 22q11.23

Cellular Component: postsynaptic membrane; protein complex; membrane; postsynaptic density; cell junction; cytosol

Molecular Function: protein serine/threonine kinase activity; protein binding; Rho guanyl-nucleotide exchange factor activity; enzyme binding; protein-tyrosine kinase activity; kinase activity; GTPase activator activity; ATP binding

Biological Process: inner ear morphogenesis; peptidyl-tyrosine phosphorylation; regulation of cell cycle; protein amino acid autophosphorylation; response to lipopolysaccharide; platelet-derived growth factor receptor signaling pathway; signal transduction; protein amino acid phosphorylation; negative regulation of neutrophil degranulation; regulation of small GTPase mediated signal transduction; small GTPase mediated signal transduction; negative regulation of inflammatory response; positive regulation of phagocytosis; brain development; neuromuscular process controlling balance; actin cytoskeleton organization and biogenesis; negative regulation of cell migration

Disease: Leukemia, Acute Lymphoblastic; Leukemia, Chronic Myeloid