AURKB sirna

AURKB siRNA (Human)

Cat. Num. AAA8229869

• Gene Names: AURKB; AIK2; AIM1; ARK2; AurB; IPL1; STK5; AIM-1; STK12; PPP1R48; aurkb-sv1; aurkb-sv2
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: AURKB; AURKB siRNA (Human); AIK2; AIM1; AIRK2; ARK2; STK1; STK12; STK5; Aurora kinase B; Aurora 1; Aurora- and IPL1-like midbody-associated protein 1; AIM-1; Aurora/IPL1-related kinase 2; ARK-2; Aurora-related kinase 2; STK-1; Serine/threonine-protein kinase 12; Serine/threonine-protein kinase 5; Serine/threonine-protein kinase aurora-B; AURKB sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: AURKB siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 303

• Applicable Applications for AURKB sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human AURKB gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for AURKB sirna

siRNA to inhibit AURKB expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 378786658
• NCBI GeneID: 9212
• NCBI Accession #: NP_001243763.1
• NCBI GenBank Nucleotide #: NM_001256834.1
• UniProt Accession #: Q96GD4; O14630; O60446; O95083; Q96DV5; Q9UQ46; B4DNM4; C7G533; C7G534; C7G535; D3DTR4; J9JID1
• Molecular Weight: 39,467 Da
• NCBI Official Full Name: aurora kinase B isoform 2
• NCBI Official Synonym Full Names: aurora kinase B
• NCBI Official Symbol: AURKB
• NCBI Official Synonym Symbols: AIK2; AIM1; ARK2; AurB; IPL1; STK5; AIM-1; STK12; PPP1R48; aurkb-sv1; aurkb-sv2
• NCBI Protein Information: aurora kinase B
• UniProt Protein Name: Aurora kinase B
• Protein Family: Aurora kinase
• UniProt Gene Name: AURKB
• UniProt Synonym Gene Names: AIK2; AIM1; AIRK2; ARK2; STK1; STK12; STK5; AIM-1; ARK-2; Aurora-related kinase 2
• UniProt Entry Name: AURKB_HUMAN

NCBI Description

This gene encodes a member of the aurora kinase subfamily of serine/threonine kinases. The genes encoding the other two members of this subfamily are located on chromosomes 19 and 20. These kinases participate in the regulation of alignment and segregation of chromosomes during mitosis and meiosis through association with microtubules. A pseudogene of this gene is located on chromosome 8. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2015]

Uniprot Description

AurB: a member of the AUR family of kinases. May be directly involved in regulating the cleavage of polar spindle microtubules and is a key regulator for the onset of cytokinesis during mitosis. Expressed during S and G2/M phase and expression is upregulated in cancer cells during M phase. Localized to the midzone of central spindle in late anaphase and concentrated into the midbody in telophase and cytokinesis. Colocalizes with gamma tubulin in the mid-body. Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Overexpressed in colorectal and other cancer cell lines and thought to cause aneuploidy via histone phosphorylation.

Protein type: Protein kinase, Ser/Thr (non-receptor); Protein kinase, Other; Kinase, protein; EC 2.7.11.1; Other group; AUR family

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: spindle microtubule; spindle; spindle midzone; spindle pole centrosome; midbody; cytosol; nucleus; condensed nuclear chromosome, pericentric region; chromocenter

Molecular Function: protein serine/threonine kinase activity; protein binding; metal ion binding; protein serine/threonine/tyrosine kinase activity; histone serine kinase activity; ATP binding

Biological Process: positive regulation of cytokinesis; spindle stabilization; spindle checkpoint; spindle midzone assembly involved in mitosis; protein amino acid autophosphorylation; regulation of chromosome segregation; negative regulation of transcription from RNA polymerase II promoter; attachment of spindle microtubules to kinetochore; protein amino acid phosphorylation; negative regulation of cytokinesis; cell proliferation; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; histone modification; abscission; mitotic cell cycle; negative regulation of protein binding; negative regulation of B cell apoptosis; aging