AURKA sirna

AURKA siRNA (Human)

Cat. Num. AAA8232838

• Gene Names: AURKA; AIK; ARK1; AURA; BTAK; STK6; STK7; STK15; AURORA2; PPP1R47
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: AURKA; AURKA siRNA (Human); AIK; AIRK1; ARK1; AURA; AYK1; BTAK; IAK1; STK15; STK6; Aurora kinase A; Aurora 2; Aurora/IPL1-related kinase 1; ARK-1; Aurora-related kinase 1; hARK1; Breast tumor-amplified kinase; Serine/threonine-protein kinase 15; Serine/threonine-protein kinase 6; Serine/threonine-protein kinase aurora-A; AURKA sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: AURKA siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 403

• Applicable Applications for AURKA sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human AURKA gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for AURKA sirna

siRNA to inhibit AURKA expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 38327562
• NCBI GeneID: 6790
• NCBI Accession #: NP_003591.2
• NCBI GenBank Nucleotide #: NM_003600.2
• UniProt Accession #: O14965; O60445; O75873; Q9BQD6; Q9UPG5; E1P5F9
• Molecular Weight: 45,809 Da
• NCBI Official Full Name: aurora kinase A
• NCBI Official Synonym Full Names: aurora kinase A
• NCBI Official Symbol: AURKA
• NCBI Official Synonym Symbols: AIK; ARK1; AURA; BTAK; STK6; STK7; STK15; AURORA2; PPP1R47
• NCBI Protein Information: aurora kinase A
• UniProt Protein Name: Aurora kinase A
• Protein Family: Aurora kinase
• UniProt Gene Name: AURKA
• UniProt Synonym Gene Names: AIK; AIRK1; ARK1; AURA; AYK1; BTAK; IAK1; STK15; STK6; ARK-1; Aurora-related kinase 1; hARK1
• UniProt Entry Name: AURKA_HUMAN

NCBI Description

The protein encoded by this gene is a cell cycle-regulated kinase that appears to be involved in microtubule formation and/or stabilization at the spindle pole during chromosome segregation. The encoded protein is found at the centrosome in interphase cells and at the spindle poles in mitosis. This gene may play a role in tumor development and progression. A processed pseudogene of this gene has been found on chromosome 1, and an unprocessed pseudogene has been found on chromosome 10. Multiple transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

AurA: a member of the AUR family of kinases. A cell cycle-regulated serine/threonine protein kinase that is overexpressed in many tumor cell lines. Regulated by phosphorylation-dependent proteasomal degradation. Localized to mitotic centrosomes and spindle microtubules and is required for centrosome maturation. Loss of Aurora A leads to defective mitotic spindles and gross errors in chromosome segregation. Required for centrosome duplication and chromosome segregation. Overexpression in culture drives transformation and aneuploidy, and negatively regulates p53. Amplified or overexpressed in many tumors or cell lines. Found as a skin tumor susceptibility gene in mouse, and a human SNP in a degradation domain is weakly cancer-associated and undergoes allele-specific amplification. Inhibitors: VX-680, ZM447439, Hesperadin, SNS-595.

Protein type: Oncoprotein; EC 2.7.11.1; Protein kinase, Other; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Other group; AUR family

Chromosomal Location of Human Ortholog: 20q13

Cellular Component: germinal vesicle; centrosome; pronucleus; spindle pole centrosome; cytosol; microtubule cytoskeleton; axon hillock; spindle microtubule; perinuclear region of cytoplasm; spindle; midbody; spindle midzone; nucleus; condensed nuclear chromosome, pericentric region

Molecular Function: protein serine/threonine kinase activity; protein binding; ubiquitin protein ligase binding; protein serine/threonine/tyrosine kinase activity; histone serine kinase activity; protein kinase binding; ATP binding; protein kinase activity

Biological Process: mitosis; spindle stabilization; regulation of centrosome cycle; positive regulation of mitosis; protein amino acid autophosphorylation; regulation of protein stability; protein amino acid phosphorylation; regulation of cytokinesis; mitotic spindle organization and biogenesis; mitotic centrosome separation; centrosome localization; cell division; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; mitotic cell cycle; G2/M transition of mitotic cell cycle; negative regulation of protein binding; spindle assembly involved in female meiosis I; negative regulation of apoptosis; anterior/posterior axis specification

Disease: Colorectal Cancer