ATP5O sirna

ATP5O siRNA (Human)

Cat. Num. AAA828012

• Gene Names: ATP5O; ATPO; OSCP; HMC08D05
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ATP5O; ATP5O siRNA (Human); ATPO; ATP synthase subunit O mitochondrial; Oligomycin sensitivity conferral protein; OSCP; ATP5O sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ATP5O siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 213

• Applicable Applications for ATP5O sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ATP5O gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ATP5O sirna

siRNA to inhibit ATP5O expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 4502303
• NCBI GeneID: 539
• NCBI Accession #: NP_001688.1
• NCBI GenBank Nucleotide #: NM_001697.2
• UniProt Accession #: P48047; Q5U042; Q6IBI2; B2R4E2
• Molecular Weight: 23,277 Da
• NCBI Official Full Name: ATP synthase subunit O, mitochondrial
• NCBI Official Synonym Full Names: ATP synthase, H+ transporting, mitochondrial F1 complex, O subunit
• NCBI Official Symbol: ATP5O
• NCBI Official Synonym Symbols: ATPO; OSCP; HMC08D05
• NCBI Protein Information: ATP synthase subunit O, mitochondrial
• UniProt Protein Name: ATP synthase subunit O, mitochondrial
• UniProt Gene Name: ATP5O
• UniProt Synonym Gene Names: ATPO; OSCP
• UniProt Entry Name: ATPO_HUMAN

NCBI Description

The protein encoded by this gene is a component of the F-type ATPase found in the mitochondrial matrix. F-type ATPases are composed of a catalytic core and a membrane proton channel. The encoded protein appears to be part of the connector linking these two components and may be involved in transmission of conformational changes or proton conductance. [provided by RefSeq, Jul 2008]

Uniprot Description

ATP5O: Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements. Belongs to the ATPase delta chain family.

Protein type: Energy Metabolism - oxidative phosphorylation; Mitochondrial; EC 3.6.3.14; Hydrolase

Chromosomal Location of Human Ortholog: 21q22.11

Cellular Component: mitochondrion; mitochondrial inner membrane; plasma membrane; nucleus; mitochondrial proton-transporting ATP synthase complex

Molecular Function: transporter activity; ATPase activity; hydrogen ion transporting ATP synthase activity, rotational mechanism; drug binding; transmembrane transporter activity

Biological Process: cellular metabolic process; proton transport; ATP biosynthetic process; mitochondrial ATP synthesis coupled proton transport