ATP2B4 sirna

ATP2B4 siRNA (Human)

Cat. Num. AAA8205198

• Gene Names: ATP2B4; MXRA1; PMCA4; ATP2B2; PMCA4b; PMCA4x
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ATP2B4; ATP2B4 siRNA (Human); ATP2B2; MXRA1; Plasma membrane calcium-transporting ATPase 4; PMCA4; Matrix-remodeling-associated protein 1; Plasma membrane calcium ATPase isoform 4; Plasma membrane calcium pump isoform 4; ATP2B4 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ATP2B4 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1170

• Applicable Applications for ATP2B4 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ATP2B4 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ATP2B4 sirna

siRNA to inhibit ATP2B4 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 48255959
• NCBI GeneID: 493
• NCBI Accession #: NP_001001396.1
• NCBI GenBank Nucleotide #: NM_001001396.2
• UniProt Accession #: P23634; Q13450; Q13452; Q13455; Q16817; Q7Z3S1; B1APW5; B1APW6
• Molecular Weight: 136,584 Da
• NCBI Official Full Name: plasma membrane calcium-transporting ATPase 4 isoform 4a
• NCBI Official Synonym Full Names: ATPase, Ca++ transporting, plasma membrane 4
• NCBI Official Symbol: ATP2B4
• NCBI Official Synonym Symbols: MXRA1; PMCA4; ATP2B2; PMCA4b; PMCA4x
• NCBI Protein Information: plasma membrane calcium-transporting ATPase 4
• UniProt Protein Name: Plasma membrane calcium-transporting ATPase 4
• Protein Family: Plasma membrane calcium-transporting ATPase
• UniProt Gene Name: ATP2B4
• UniProt Synonym Gene Names: ATP2B2; MXRA1; PMCA4
• UniProt Entry Name: AT2B4_HUMAN

NCBI Description

The protein encoded by this gene belongs to the family of P-type primary ion transport ATPases characterized by the formation of an aspartyl phosphate intermediate during the reaction cycle. These enzymes remove bivalent calcium ions from eukaryotic cells against very large concentration gradients and play a critical role in intracellular calcium homeostasis. The mammalian plasma membrane calcium ATPase isoforms are encoded by at least four separate genes and the diversity of these enzymes is further increased by alternative splicing of transcripts. The expression of different isoforms and splice variants is regulated in a developmental, tissue- and cell type-specific manner, suggesting that these pumps are functionally adapted to the physiological needs of particular cells and tissues. This gene encodes the plasma membrane calcium ATPase isoform 4. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

PMCA4: a plasma membrane calcium pump. A magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium out of the cell. Eight splice-variant isoforms have been described.

Protein type: Membrane protein, multi-pass; EC 3.6.3.8; Transporter; Membrane protein, integral; Transporter, ion channel; Hydrolase; Channel, calcium

Chromosomal Location of Human Ortholog: 1q32.1

Cellular Component: protein complex; membrane; integral to plasma membrane; T-tubule; plasma membrane; caveola; Z disc

Molecular Function: calmodulin binding; protein binding; sodium channel regulator activity; calcium-transporting ATPase activity; metal ion binding; nitric-oxide synthase binding; ATP binding; protein phosphatase 2B binding

Biological Process: regulation of transcription from RNA polymerase II promoter; calcium ion homeostasis; cellular calcium ion homeostasis; negative regulation of nitric-oxide synthase activity; metabolic process; negative regulation of nitric oxide biosynthetic process; blood coagulation; response to hydrostatic pressure; transmembrane transport; positive regulation of peptidyl-serine phosphorylation