ASPM sirna

ASPM siRNA (Human)

Cat. Num. AAA8202961

• Gene Names: ASPM; ASP; MCPH5; Calmbp1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ASPM; ASPM siRNA (Human); MCPH5; Abnormal spindle-like microcephaly-associated protein; Abnormal spindle protein homolog; Asp homolog; ASPM sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ASPM siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1892

• Applicable Applications for ASPM sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ASPM gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ASPM sirna

siRNA to inhibit ASPM expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 332205965
• NCBI GeneID: 259266
• NCBI Accession #: NP_001193775.1
• NCBI GenBank Nucleotide #: NM_001206846.1
• UniProt Accession #: Q8IZT6; Q4G1H1; Q5VYL3; Q86UX4; Q8IUL2; Q8IZJ7; Q8IZJ8; Q8IZJ9; Q8N4D1; Q9NVS1; Q9NVT6
• Molecular Weight: 217,794 Da
• NCBI Official Full Name: abnormal spindle-like microcephaly-associated protein isoform 2
• NCBI Official Synonym Full Names: abnormal spindle microtubule assembly
• NCBI Official Symbol: ASPM
• NCBI Official Synonym Symbols: ASP; MCPH5; Calmbp1
• NCBI Protein Information: abnormal spindle-like microcephaly-associated protein
• UniProt Protein Name: Abnormal spindle-like microcephaly-associated protein
• Protein Family: Abnormal spindle-like microcephaly-associated protein
• UniProt Gene Name: ASPM
• UniProt Synonym Gene Names: MCPH5; Asp homolog
• UniProt Entry Name: ASPM_HUMAN

NCBI Description

This gene is the human ortholog of the Drosophila melanogaster 'abnormal spindle' gene (asp), which is essential for normal mitotic spindle function in embryonic neuroblasts. Studies in mouse also suggest a role of this gene in mitotic spindle regulation, with a preferential role in regulating neurogenesis. Mutations in this gene are associated with microcephaly primary type 5. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, May 2011]

Uniprot Description

ASPM: Probable role in mitotic spindle regulation and coordination of mitotic processes. May have a preferential role in regulating neurogenesis. Defects in ASPM are the cause of microcephaly primary type 5 (MCPH5); also known as true microcephaly or microcephaly vera. Microcephaly is defined as a head circumference more than 3 standard deviations below the age-related mean. Brain weight is markedly reduced and the cerebral cortex is disproportionately small. Despite this marked reduction in size, the gyral pattern is relatively well preserved, with no major abnormality in cortical architecture. Primary microcephaly is further defined by the absence of other syndromic features or significant neurological deficits. This entity is inherited as autosomal recessive trait. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation

Chromosomal Location of Human Ortholog: 1q31

Cellular Component: microtubule; cytoplasm; midbody; nucleus

Molecular Function: calmodulin binding

Biological Process: oogenesis; positive regulation of neuroblast proliferation; developmental growth; mitosis; maintenance of centrosome localization; negative regulation of neuron differentiation; negative regulation of asymmetric cell division; male gonad development; neuron migration; spermatogenesis; cerebral cortex development; forebrain neuroblast division

Disease: Microcephaly 5, Primary, Autosomal Recessive