ASNS sirna

ASNS siRNA (Human)

Cat. Num. AAA8240620

• Gene Names: ASNS; TS11; ASNSD
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ASNS; ASNS siRNA (Human); TS11; Asparagine synthetase [glutamine-hydrolyzing]; Cell cycle control protein TS11; Glutamine-dependent asparagine synthetase; ASNS sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ASNS siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 540

• Applicable Applications for ASNS sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ASNS gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ASNS sirna

siRNA to inhibit ASNS expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 296010848
• NCBI GeneID: 440
• NCBI Accession #: NP_001171546.1
• NCBI GenBank Nucleotide #: NM_001178075.1
• UniProt Accession #: P08243; P08184; Q15666; Q549T9; Q96HD0; A4D1I8; B4DXZ1; B7ZAA9; D6W5R3; E9PCI3; E9PCX6
• Molecular Weight: 54,818 Da
• NCBI Official Full Name: asparagine synthetase
• NCBI Official Synonym Full Names: asparagine synthetase (glutamine-hydrolyzing)
• NCBI Official Symbol: ASNS
• NCBI Official Synonym Symbols: TS11; ASNSD
• NCBI Protein Information: asparagine synthetase [glutamine-hydrolyzing]
• UniProt Protein Name: Asparagine synthetase [glutamine-hydrolyzing]
• Protein Family: Asparagine--tRNA ligase
• UniProt Gene Name: ASNS
• UniProt Synonym Gene Names: TS11
• UniProt Entry Name: ASNS_HUMAN

NCBI Description

The protein encoded by this gene is involved in the synthesis of asparagine. This gene complements a mutation in the temperature-sensitive hamster mutant ts11, which blocks progression through the G1 phase of the cell cycle at nonpermissive temperature. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, May 2010]

Uniprot Description

ASNS: 3 isoforms of the human protein are produced by alternative splicing

Protein type: Ligase; Amino Acid Metabolism - alanine, aspartate and glutamate; Cell cycle regulation; EC 6.3.5.4; Energy Metabolism - nitrogen

Chromosomal Location of Human Ortholog: 7q21.3

Cellular Component: cytosol

Molecular Function: protein homodimerization activity; asparagine synthase (glutamine-hydrolyzing) activity; cofactor binding; ATP binding

Biological Process: response to light stimulus; unfolded protein response; response to toxin; glutamine metabolic process; positive regulation of mitotic cell cycle; liver development; response to amino acid stimulus; cellular response to hormone stimulus; cellular response to glucose starvation; cellular protein metabolic process; asparagine biosynthetic process; unfolded protein response, activation of signaling protein activity; response to mechanical stimulus; amino acid biosynthetic process; response to methotrexate; response to follicle-stimulating hormone stimulus; negative regulation of apoptosis

Disease: Asparagine Synthetase Deficiency