ASAP2 sirna

ASAP2 siRNA (Human)

Cat. Num. AAA8232959

• Gene Names: ASAP2; PAP; PAG3; AMAP2; DDEF2; SHAG1; CENTB3; Pap-alpha
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ASAP2; ASAP2 siRNA (Human); DDEF2; KIAA0400; Arf-GAP with SH3 domain. ANK repeat and PH domain-containing protein 2; Development and differentiation-enhancing factor 2; Paxillin-associated protein with ARF GAP activity 3; PAG3; Pyk2 C-terminus-associated protein; PAP; ASAP2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ASAP2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 961

• Applicable Applications for ASAP2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ASAP2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ASAP2 sirna

siRNA to inhibit ASAP2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 206597522
• NCBI GeneID: 8853
• NCBI Accession #: NP_001128663.1
• NCBI GenBank Nucleotide #: NM_001135191.1
• UniProt Accession #: O43150; D6W4Y8
• Molecular Weight: 106,982 Da
• NCBI Official Full Name: arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2 isoform b
• NCBI Official Synonym Full Names: ArfGAP with SH3 domain, ankyrin repeat and PH domain 2
• NCBI Official Symbol: ASAP2
• NCBI Official Synonym Symbols: PAP; PAG3; AMAP2; DDEF2; SHAG1; CENTB3; Pap-alpha
• NCBI Protein Information: arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2
• UniProt Protein Name: Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2
• Protein Family: Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein
• UniProt Gene Name: ASAP2
• UniProt Synonym Gene Names: DDEF2; KIAA0400; PAG3; PAP
• UniProt Entry Name: ASAP2_HUMAN

NCBI Description

This gene encodes a multidomain protein containing an N-terminal alpha-helical region with a coiled-coil motif, followed by a pleckstrin homology (PH) domain, an Arf-GAP domain, an ankyrin homology region, a proline-rich region, and a C-terminal Src homology 3 (SH3) domain. The protein localizes in the Golgi apparatus and at the plasma membrane, where it colocalizes with protein tyrosine kinase 2-beta (PYK2). The encoded protein forms a stable complex with PYK2 in vivo. This interaction appears to be mediated by binding of its SH3 domain to the C-terminal proline-rich domain of PYK2. The encoded protein is tyrosine phosphorylated by activated PYK2. It has catalytic activity for class I and II ArfGAPs in vitro, and can bind the class III Arf ARF6 without immediate GAP activity. The encoded protein is believed to function as an ARF GAP that controls ARF-mediated vesicle budding when recruited to Golgi membranes. In addition, it functions as a substrate and downstream target for PYK2 and SRC, a pathway that may be involved in the regulation of vesicular transport. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008]

Uniprot Description

DDEF2: Activates the small GTPases ARF1, ARF5 and ARF6. Regulates the formation of post-Golgi vesicles and modulates constitutive secretion. Modulates phagocytosis mediated by Fc gamma receptor and ARF6. Modulates PXN recruitment to focal contacts and cell migration. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: GAPs; GAPs, ARF; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 2p25|2p24

Cellular Component: plasma membrane

Molecular Function: protein binding; zinc ion binding; enzyme activator activity

Biological Process: positive regulation of catalytic activity; positive regulation of GTPase activity