ARRB1 sirna

ARRB1 siRNA (Human)

Cat. Num. AAA8209500

• Gene Names: ARRB1; ARB1; ARR1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ARRB1; ARRB1 siRNA (Human); ARR1; Beta-arrestin-1; Arrestin beta-1; ARRB1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ARRB1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 418

• Applicable Applications for ARRB1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ARRB1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ARRB1 sirna

siRNA to inhibit ARRB1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 10880136
• NCBI GeneID: 408
• NCBI Accession #: NP_004032.2
• NCBI GenBank Nucleotide #: NM_004041.4
• UniProt Accession #: P49407; O75625; O75630; Q2PP20; Q9BTK8; B6V9G8
• Molecular Weight: 46,309 Da
• NCBI Official Full Name: beta-arrestin-1 isoform A
• NCBI Official Synonym Full Names: arrestin, beta 1
• NCBI Official Symbol: ARRB1
• NCBI Official Synonym Symbols: ARB1; ARR1
• NCBI Protein Information: beta-arrestin-1
• UniProt Protein Name: Beta-arrestin-1
• Protein Family: Beta-arrestin
• UniProt Gene Name: ARRB1
• UniProt Synonym Gene Names: ARR1
• UniProt Entry Name: ARRB1_HUMAN

NCBI Description

Members of arrestin/beta-arrestin protein family are thought to participate in agonist-mediated desensitization of G-protein-coupled receptors and cause specific dampening of cellular responses to stimuli such as hormones, neurotransmitters, or sensory signals. Arrestin beta 1 is a cytosolic protein and acts as a cofactor in the beta-adrenergic receptor kinase (BARK) mediated desensitization of beta-adrenergic receptors. Besides the central nervous system, it is expressed at high levels in peripheral blood leukocytes, and thus the BARK/beta-arrestin system is believed to play a major role in regulating receptor-mediated immune functions. Alternatively spliced transcripts encoding different isoforms of arrestin beta 1 have been described. [provided by RefSeq, Jan 2011]

Uniprot Description

ARRB1: regulates G-protein coupled receptors (GPCR) signaling by mediating both receptor desensitization and resensitization processes. Binds to GRK-phosphorylated receptor and sterically preclude its coupling to the cognate G- protein; the binding appears to require receptor determinants exposed only in the active receptor conformation. Targets many receptors for internalization by acting as endocytic adapters (CLASPs, clathrin-associated sorting proteins). Internalized arrestin-receptor complexes traffic to intracellular endosomes, where they remain uncoupled from G-proteins. Two different modes of arrestin-mediated internalization occur. Beta-arrestins function as multivalent adapter proteins that can switch the GPCR from a G-protein signaling mode that transmits short-lived signals from the plasma membrane via small molecule second messengers and ion channels to a beta-arrestin signaling mode that transmits a distinct set of signals that are initiated as the receptor internalizes and transits the intracellular compartment. Also involved in regulation of receptors other than GPCRs. Involved in Toll-like receptor and IL-1 receptor signaling through the interaction with TRAF6 which prevents TRAF6 autoubiquitination and oligomerization required for activation of NF-kappa-B and JUN. Binds phosphoinositides. Binds inositolhexakisphosphate (InsP6). Involved in IL8-mediated granule release in neutrophils. Interacts with phosphorylated ADRB2 and CHRM2. Interacts with SRC (via the SH3 domain and the protein kinase domain); the interaction is independent of the phosphorylation state of SRC C-terminus. Interacts with RAF1, CHUK, IKBKB and Nik. Interacts with DVL1 and DVL2; the interaction is enhanced by DVL phosphorylation. Interacts with IGF1R. Belongs to the arrestin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: Golgi membrane; nucleoplasm; cytoplasmic vesicle membrane; lysosomal membrane; cytoplasm; plasma membrane; heterotrimeric G-protein complex; coated pit; pseudopodium; cytoplasmic vesicle; chromatin; nucleus; cytosol

Molecular Function: angiotensin receptor binding; enzyme inhibitor activity; insulin-like growth factor receptor binding; protein binding; histone acetyltransferase activity; mitogen-activated protein kinase kinase binding; ubiquitin protein ligase binding; caspase inhibitor activity; transcription factor binding; GTPase activator activity

Biological Process: transcription from RNA polymerase II promoter; proteasomal ubiquitin-dependent protein catabolic process; platelet activation; Notch signaling pathway; positive regulation of protein binding; positive regulation of receptor internalization; protein ubiquitination; positive regulation of Rho protein signal transduction; negative regulation of caspase activity; positive regulation of peptidyl-serine phosphorylation; protein transport; G-protein coupled receptor internalization; inhibition of NF-kappaB transcription factor; negative regulation of interleukin-6 production; phototransduction; negative regulation of interleukin-8 production; stress fiber formation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of histone acetylation; negative regulation of protein ubiquitination; post-Golgi vesicle-mediated transport; positive regulation of protein amino acid phosphorylation; blood coagulation; positive regulation of GTPase activity