ARPC2 sirna

ARPC2 siRNA (Human)

Cat. Num. AAA8206342

• Gene Names: ARPC2; ARC34; PRO2446; p34-Arc; PNAS-139
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ARPC2; ARPC2 siRNA (Human); ARC34; Actin-related protein 2/3 complex subunit 2; Arp2/3 complex 34 kDa subunit; p34-ARC; ARPC2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ARPC2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 300

• Applicable Applications for ARPC2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ARPC2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ARPC2 sirna

siRNA to inhibit ARPC2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 5031599
• NCBI GeneID: 10109
• NCBI Accession #: NP_005722.1
• NCBI GenBank Nucleotide #: NM_005731.3
• UniProt Accession #: O15144; Q92801; Q9P1D4
• Molecular Weight: 34,333 Da
• NCBI Official Full Name: actin-related protein 2/3 complex subunit 2
• NCBI Official Synonym Full Names: actin related protein 2/3 complex, subunit 2, 34kDa
• NCBI Official Symbol: ARPC2
• NCBI Official Synonym Symbols: ARC34; PRO2446; p34-Arc; PNAS-139
• NCBI Protein Information: actin-related protein 2/3 complex subunit 2
• UniProt Protein Name: Actin-related protein 2/3 complex subunit 2
• UniProt Gene Name: ARPC2
• UniProt Synonym Gene Names: ARC34; p34-ARC
• UniProt Entry Name: ARPC2_HUMAN

NCBI Description

This gene encodes one of seven subunits of the human Arp2/3 protein complex. The Arp2/3 protein complex has been implicated in the control of actin polymerization in cells and has been conserved through evolution. The exact role of the protein encoded by this gene, the p34 subunit, has yet to be determined. Two alternatively spliced variants have been characterized to date. Additional alternatively spliced variants have been described but their full length nature has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

ARPC2: Functions as actin-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the mother actin filament. Belongs to the ARPC2 family.

Protein type: Motility/polarity/chemotaxis; Actin-binding; Cytoskeletal

Chromosomal Location of Human Ortholog: 2q36.1

Cellular Component: Golgi apparatus; Arp2/3 protein complex; neuron projection; focal adhesion; cytoplasm; leading edge; plasma membrane; synapse; cytosol; endosome; actin cytoskeleton

Molecular Function: actin filament binding; protein binding; structural constituent of cytoskeleton; kinesin binding

Biological Process: axon guidance; ephrin receptor signaling pathway; innate immune response; cell motility