ARNTL sirna

ARNTL siRNA (Human)

Cat. Num. AAA8229748

• Gene Names: ARNTL; TIC; JAP3; MOP3; BMAL1; PASD3; BMAL1c; bHLHe5
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ARNTL; ARNTL siRNA (Human); BHLHE5; BMAL1; MOP3; PASD3; Aryl hydrocarbon receptor nuclear translocator-like protein 1; Basic-helix-loop-helix-PAS protein MOP3; Brain and muscle ARNT-like 1; Class E basic helix-loop-helix protein 5; bHLHe5; Member of PAS protein 3; PAS domain-containing protein 3; bHLH-PAS protein JAP3; ARNTL sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ARNTL siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 625

• Applicable Applications for ARNTL sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ARNTL gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ARNTL sirna

siRNA to inhibit ARNTL expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 71852580
• NCBI GeneID: 406
• NCBI Accession #: NP_001025443.1
• NCBI GenBank Nucleotide #: NM_001030272.2
• UniProt Accession #: O00327; O00313; O00314; O00315; O00316; O00317; Q4G136; A2I2N6; A8K645; B5ME11; B7WPG7; D3DQW6
• Molecular Weight: 64,136 Da
• NCBI Official Full Name: aryl hydrocarbon receptor nuclear translocator-like protein 1 isoform a
• NCBI Official Synonym Full Names: aryl hydrocarbon receptor nuclear translocator-like
• NCBI Official Symbol: ARNTL
• NCBI Official Synonym Symbols: TIC; JAP3; MOP3; BMAL1; PASD3; BMAL1c; bHLHe5
• NCBI Protein Information: aryl hydrocarbon receptor nuclear translocator-like protein 1
• UniProt Protein Name: Aryl hydrocarbon receptor nuclear translocator-like protein 1
• Protein Family: Aryl hydrocarbon receptor nuclear translocator-like protein
• UniProt Gene Name: ARNTL
• UniProt Synonym Gene Names: BHLHE5; BMAL1; MOP3; PASD3; bHLHe5
• UniProt Entry Name: BMAL1_HUMAN

NCBI Description

The protein encoded by this gene is a basic helix-loop-helix protein that forms a heterodimer with CLOCK. This heterodimer binds E-box enhancer elements upstream of Period (PER1, PER2, PER3) and Cryptochrome (CRY1, CRY2) genes and activates transcription of these genes. PER and CRY proteins heterodimerize and repress their own transcription by interacting in a feedback loop with CLOCK/ARNTL complexes. Defects in this gene have been linked to infertility, problems with gluconeogenesis and lipogenesis, and altered sleep patterns. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014]

Uniprot Description

BMAL1: aryl hydrocarbon receptor nuclear translocator-like protein. A circadian regulatory protein and substrate of casein kinase I epsilon. Heterodimers bind to an E-box element (3'-CACGTG-5'), thereby activating transcription of PER1, and possibly of other circadian clock proteins. Seven splice isoforms have been reported.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 11p15

Cellular Component: PML body; transcription factor complex; nucleus

Molecular Function: protein binding; signal transducer activity; DNA binding; aryl hydrocarbon receptor binding; sequence-specific DNA binding; protein heterodimerization activity; bHLH transcription factor binding; Hsp90 protein binding

Biological Process: transcription from RNA polymerase II promoter; circadian rhythm; proteasomal ubiquitin-dependent protein catabolic process; negative regulation of TOR signaling pathway; regulation of cell cycle; positive regulation of transcription, DNA-dependent; response to redox state; positive regulation of circadian rhythm; signal transduction; negative regulation of fat cell differentiation; regulation of neurogenesis; regulation of transcription, DNA-dependent; protein import into nucleus, translocation; positive regulation of transcription from RNA polymerase II promoter; spermatogenesis; regulation of protein catabolic process; circadian regulation of gene expression; negative regulation of transcription, DNA-dependent; regulation of insulin secretion; regulation of hair cycle