APPL2 sirna

APPL2 siRNA (Human)

Cat. Num. AAA8226216

• Gene Names: APPL2; DIP13B
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: APPL2; APPL2 siRNA (Human); DIP13B; DCC-interacting protein 13-beta; Dip13-beta; Adapter protein containing PH domain. PTB domain and leucine zipper motif 2; APPL2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: APPL2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 670

• Applicable Applications for APPL2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human APPL2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for APPL2 sirna

siRNA to inhibit APPL2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 353731084
• NCBI GeneID: 55198
• NCBI Accession #: NP_001238833.1
• NCBI GenBank Nucleotide #: NM_001251904.1
• UniProt Accession #: Q8NEU8; Q8N4R7; Q9NVL2; B7Z411; B7Z4B0; F5GZG0; F8W1P5
• Molecular Weight: 75,184 Da
• NCBI Official Full Name: DCC-interacting protein 13-beta isoform 2
• NCBI Official Synonym Full Names: adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 2
• NCBI Official Symbol: APPL2
• NCBI Official Synonym Symbols: DIP13B
• NCBI Protein Information: DCC-interacting protein 13-beta
• UniProt Protein Name: DCC-interacting protein 13-beta
• Protein Family: DCC-interacting protein
• UniProt Gene Name: APPL2
• UniProt Synonym Gene Names: DIP13B; Dip13-beta
• UniProt Entry Name: DP13B_HUMAN

NCBI Description

The protein encoded by this gene is one of two effectors of the small GTPase RAB5A/Rab5, which are involved in a signal transduction pathway. Both effectors contain an N-terminal Bin/Amphiphysin/Rvs (BAR) domain, a central pleckstrin homology (PH) domain, and a C-terminal phosphotyrosine binding (PTB) domain, and they bind the Rab5 through the BAR domain. They are associated with endosomal membranes and can be translocated to the nucleus in response to the EGF stimulus. They interact with the NuRD/MeCP1 complex (nucleosome remodeling and deacetylase /methyl-CpG-binding protein 1 complex) and are required for efficient cell proliferation. A chromosomal aberration t(12;22)(q24.1;q13.3) involving this gene and the PSAP2 gene results in 22q13.3 deletion syndrome, also known as Phelan-McDermid syndrome. [provided by RefSeq, Oct 2011]

Uniprot Description

APPL2: a Rab5 effector protein that resides on a subpopulation of endosomes. Required for the regulation of cell proliferation in response to extracellular signals mediated by an early endosomal compartment. Links Rab5 to nuclear signal transduction. Its function requires Rab5 binding. Translocated into the nucleus upon release from endosomal membranes following internalization of EGF. Binds to subunits of the the nucleosome remodeling and deacetylase (NuRD) complex, an abundant and widely expressed deacetylase complex. The NURD complex contains both histone deacetylation and chromatin remodeling ATPase activities. Contains a PH domain and a phosphotyrosine interaction domain (PID) domain that has a structure similar to the insulin receptor substrate-1 PTB domain.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 12q24.1

Cellular Component: early endosome membrane; cytoplasm; endosome membrane; NuRD complex; nucleus

Molecular Function: protein binding

Biological Process: cell proliferation; cell cycle; signal transduction