APOBEC3F sirna

APOBEC3F siRNA (Human)

Cat. Num. AAA8224142

• Gene Names: APOBEC3F; A3F; KA6; ARP8; BK150C2.4.MRNA
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: APOBEC3F; APOBEC3F siRNA (Human); DNA dC->dU-editing enzyme APOBEC-3F; Apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 3F; A3F; APOBEC3F sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: APOBEC3F siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 101

• Applicable Applications for APOBEC3F sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human APOBEC3F gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for APOBEC3F sirna

siRNA to inhibit APOBEC3F expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 54873619
• NCBI GeneID: 200316
• NCBI Accession #: NP_001006667.1
• NCBI GenBank Nucleotide #: NM_001006666.1
• UniProt Accession #: Q8IUX4; Q45F03; Q6ICH3; Q7Z2N2; Q7Z2N5; B0QYD4
• Molecular Weight: 11,823 Da
• NCBI Official Full Name: DNA dC->dU-editing enzyme APOBEC-3F isoform b
• NCBI Official Synonym Full Names: apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3F
• NCBI Official Symbol: APOBEC3F
• NCBI Official Synonym Symbols: A3F; KA6; ARP8; BK150C2.4.MRNA
• NCBI Protein Information: DNA dC->dU-editing enzyme APOBEC-3F
• UniProt Protein Name: DNA dC->dU-editing enzyme APOBEC-3F
• Protein Family: DNA dC->dU-editing enzyme
• UniProt Gene Name: APOBEC3F
• UniProt Synonym Gene Names: A3F
• UniProt Entry Name: ABC3F_HUMAN

NCBI Description

This gene is a member of the cytidine deaminase gene family. It is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22. Members of the cluster encode proteins that are structurally and functionally related to the C to U RNA-editing cytidine deaminase APOBEC1. It is thought that the proteins may be RNA editing enzymes and have roles in growth or cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

APOBEC3F: Host cellular restriction factor that may have antiviral activities against exogenous and endogenous viruses, as well as retrotransposons. After being packaged into HIV-1 virions, blocks productive infection by massively editing dC residues to dU on the DNA minus strand during reverse transcription. The editing of the minus strand DNA of HIV-1 during reverse transcription leads to G- to-A transitions in the plus strand. The inhibition of viral replication is either due to the degradation of the minus strand before its integration or to the lethality of the hypermutations. May also play a role in the epigenetic regulation of gene expression through the process of active DNA demethylation. Belongs to the cytidine and deoxycytidylate deaminase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA processing; Hydrolase; EC 3.5.4.-

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: cytoplasm; apolipoprotein B mRNA editing enzyme complex; ribonucleoprotein complex

Molecular Function: protein binding; zinc ion binding; RNA binding; cytidine deaminase activity

Biological Process: negative regulation of retroviral genome replication; base conversion or substitution editing; negative regulation of viral genome replication; innate immune response; cytidine deamination; positive regulation of defense response to virus by host; defense response to virus; negative regulation of viral reproduction