APOBEC3A sirna

APOBEC3A siRNA (Human)

Cat. Num. AAA8209871

• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: APOBEC3A; APOBEC3A siRNA (Human); DNA dC->dU-editing enzyme APOBEC-3A; A3A; Phorbolin-1; APOBEC3A sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: APOBEC3A siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 199

• Applicable Applications for APOBEC3A sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human APOBEC3A gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for APOBEC3A sirna

siRNA to inhibit APOBEC3A expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 300869638
• NCBI GeneID: 100913187
• NCBI Accession #: NP_001180218.1
• NCBI GenBank Nucleotide #: NM_001193289.1
• UniProt Accession #: P31941; Q12807; Q5JZ93; Q9UH18; A0AVM1
• Molecular Weight: 21,778 Da
• NCBI Official Full Name: probable DNA dC->dU-editing enzyme APOBEC-3A
• NCBI Official Synonym Full Names: APOBEC3A and APOBEC3B deletion hybrid
• NCBI Official Symbol: APOBEC3A_B
• NCBI Protein Information: probable DNA dC->dU-editing enzyme APOBEC-3A
• UniProt Protein Name: DNA dC->dU-editing enzyme APOBEC-3A
• Protein Family: DNA dC->dU-editing enzyme
• UniProt Gene Name: APOBEC3A
• UniProt Synonym Gene Names: A3A
• UniProt Entry Name: ABC3A_HUMAN

NCBI Description

This gene is a member of the cytidine deaminase gene family. It is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22. Members of the cluster encode proteins that are structurally and functionally related to the C to U RNA-editing cytidine deaminase APOBEC1. The protein encoded by this gene lacks the zinc binding activity of other family members. The protein plays a role in immunity, by restricting transmission of foreign DNA such as viruses. One mechanism of foreign DNA restriction is deamination of foreign double-stranded DNA cytidines to uridines, which leads to DNA degradation. However, other mechanisms are also thought to be involved, as anti-viral effect is not dependent on deaminase activity. The protein encoded by this gene is the same as that encoded by APOBEC3A; however, this gene is a hybrid gene that results from the deletion of approximately 29.5 kb of sequence between the APOBEC3A gene and the adjacent gene APOBEC3B. The breakpoints of the deletion are within the two genes, so the deletion hybrid is predicted to have the promoter and coding region of APOBEC3A, but the 3' UTR of APOBEC3B. [provided by RefSeq, Jul 2012]

Uniprot Description

APOBEC3A: a single-stranded DNA cytidine deaminase involved in foreign DNA clearance with restriction activity against viruses, foreign DNA and mobility of retrotransposons. Exhibits antiviral activity against adeno-associated virus (AAV) and human T-cell leukemia virus type 1 (HTLV-1) and may inhibit the mobility of LTR and non-LTR retrotransposons. Selectively targets single-stranded DNA and can deaminate both methylcytosine and cytosine in foreign DNA. Can induce somatic C-to-U hypermutation in nuclear and mitochondrial DNA. May also play a role in the epigenetic regulation of gene expression through the process of active DNA demethylation. Interacts with AGO2. Interacts with TRIB3. Expressed in peripheral leukocytes with higher expression in CD14-positive phagocytic cells. Highly expressed in keratinocytes and in periphery blood monocytes. Also detected in non-lymphoid tissues including lung and adipose tissues. Found at high levels in colorectal adenocarcinoma, Burkitt's lymphoma and chronic myelogenous leukemia. Up-regulated by interferon and CpG single-stranded DNA (at protein level). Belongs to the cytidine and deoxycytidylate deaminase family. 2 isoforms of the human protein are produced by alternative initiation.

Protein type: EC 3.5.4.-; Hydrolase

Chromosomal Location of Human Ortholog: 22q13.1-q13.2

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: protein binding; deoxycytidine deaminase activity; zinc ion binding; cytidine deaminase activity

Biological Process: negative regulation of viral genome replication; innate immune response; cytidine deamination; defense response to virus