AP1S2 sirna

AP1S2 siRNA (Human)

Cat. Num. AAA8224185

• Gene Names: AP1S2; PGS; DC22; MRX59; MRXS5; MRXSF; MRXS21; SIGMA1B
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: AP1S2; AP1S2 siRNA (Human); AP-1 complex subunit sigma-2; Adapter-related protein complex 1 subunit sigma-1B; Adaptor protein complex AP-1 subunit sigma-1B; Clathrin assembly protein complex 1 sigma-1B small chain; Golgi adaptor HA1/AP1 adaptin sigma-1B subunit; Sigma 1B subunit of AP-1 clathrin; Sigma-adaptin 1B; Sigma1B-adaptin; AP1S2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: AP1S2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 157

• Applicable Applications for AP1S2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human AP1S2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for AP1S2 sirna

siRNA to inhibit AP1S2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 22027655
• NCBI GeneID: 8905
• NCBI Accession #: NP_003907.3
• NCBI GenBank Nucleotide #: NM_003916.4
• UniProt Accession #: P56377; O95326; Q9H2N6; B4DSU4
• Molecular Weight: 23,225 Da
• NCBI Official Full Name: AP-1 complex subunit sigma-2 isoform 2
• NCBI Official Synonym Full Names: adaptor-related protein complex 1, sigma 2 subunit
• NCBI Official Symbol: AP1S2
• NCBI Official Synonym Symbols: PGS; DC22; MRX59; MRXS5; MRXSF; MRXS21; SIGMA1B
• NCBI Protein Information: AP-1 complex subunit sigma-2
• UniProt Protein Name: AP-1 complex subunit sigma-2
• UniProt Gene Name: AP1S2
• UniProt Entry Name: AP1S2_HUMAN

NCBI Description

Adaptor protein complex 1 is found at the cytoplasmic face of coated vesicles located at the Golgi complex, where it mediates both the recruitment of clathrin to the membrane and the recognition of sorting signals within the cytosolic tails of transmembrane receptors. This complex is a heterotetramer composed of two large, one medium, and one small adaptin subunit. The protein encoded by this gene serves as the small subunit of this complex and is a member of the adaptin protein family. Transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2013]

Uniprot Description

AP1S2: Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules. Defects in AP1S2 are the cause of mental retardation X- linked type 59 (MRX59). It is characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. In contrast to syndromic or specific X-linked mental retardation which also present with associated physical, neurological and/or psychiatric manifestations, intellectual deficiency is the only primary symptom of non-syndromic X-linked mental retardation. MRX59 consists of a mild-to-profound mental retardation. Other features includes hypotonia early in life and delay in walking. Belongs to the adaptor complexes small subunit family.

Chromosomal Location of Human Ortholog: Xp22.2

Cellular Component: Golgi membrane; cytoplasmic vesicle membrane; lysosomal membrane; AP-type membrane coat adaptor complex; trans-Golgi network membrane; coated pit; cytosol

Molecular Function: protein transporter activity

Biological Process: intracellular protein transport; viral reproduction; antigen processing and presentation of exogenous peptide antigen via MHC class II; post-Golgi vesicle-mediated transport; regulation of defense response to virus by virus

Disease: Mental Retardation, X-linked, Syndromic 5