AOC1 sirna

AOC1 siRNA (Human)

Cat. Num. AAA8205259

• Gene Names: AOC1; ABP; DAO; KAO; ABP1; DAO1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: AOC1; AOC1 siRNA (Human); ABP1; DAO1; Amiloride-sensitive amine oxidase [copper-containing]; DAO; Diamine oxidase; Amiloride-binding protein 1; Amine oxidase copper domain-containing protein 1; Histaminase; Kidney amine oxidase; KAO; AOC1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: AOC1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 751

• Applicable Applications for AOC1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human AOC1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for AOC1 sirna

siRNA to inhibit AOC1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 73486661
• NCBI GeneID: 26
• NCBI Accession #: NP_001082.2
• NCBI GenBank Nucleotide #: NM_001091.3
• UniProt Accession #: P19801; Q16683; Q16684; Q56II4; Q6GU42; C9J690
• Molecular Weight: 87,239 Da
• NCBI Official Full Name: amiloride-sensitive amine oxidase
• NCBI Official Synonym Full Names: amine oxidase, copper containing 1
• NCBI Official Symbol: AOC1
• NCBI Official Synonym Symbols: ABP; DAO; KAO; ABP1; DAO1
• NCBI Protein Information: amiloride-sensitive amine oxidase [copper-containing]
• UniProt Protein Name: Amiloride-sensitive amine oxidase [copper-containing]
• Protein Family: Allene oxide cyclase
• UniProt Gene Name: AOC1
• UniProt Synonym Gene Names: ABP1; DAO1; DAO; Diamine oxidase; KAO
• UniProt Entry Name: AOC1_HUMAN

NCBI Description

This gene encodes a metal-binding membrane glycoprotein that oxidatively deaminates putrescine, histamine, and related compounds. The encoded protein is inhibited by amiloride, a diuretic that acts by closing epithelial sodium ion channels. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2013]

Uniprot Description

ABP1: Catalyzes the degradation of compounds such as putrescine, histamine, spermine, and spermidine, substances involved in allergic and immune responses, cell proliferation, tissue differentiation, tumor formation, and possibly apoptosis. Placental DAO is thought to play a role in the regulation of the female reproductive function. Belongs to the copper/topaquinone oxidase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Amino Acid Metabolism - tryptophan; Secreted, signal peptide; Amino Acid Metabolism - arginine and proline; Secreted; Oxidoreductase; Amino Acid Metabolism - histidine; EC 1.4.3.22

Chromosomal Location of Human Ortholog: 7q36.1

Cellular Component: extracellular space; tight junction; plasma membrane; peroxisome

Molecular Function: heparin binding; amine oxidase activity; protein homodimerization activity; copper ion binding; zinc ion binding; protein complex binding; calcium ion binding; drug binding; cation channel activity; receptor activity; sodium channel activity; quinone binding

Biological Process: response to drug; response to antibiotic; amine metabolic process