ALMS1 sirna

ALMS1 siRNA (Human)

Cat. Num. AAA8239262

• Gene Names: ALMS1; ALSS
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ALMS1; ALMS1 siRNA (Human); KIAA0328; Alstrom syndrome protein 1; ALMS1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ALMS1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 4169

• Applicable Applications for ALMS1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ALMS1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ALMS1 sirna

siRNA to inhibit ALMS1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 110349786
• NCBI GeneID: 7840
• NCBI Accession #: NP_055935.4
• NCBI GenBank Nucleotide #: NM_015120.4
• UniProt Accession #: Q8TCU4; Q53S05; Q580Q8; Q86VP9; Q9Y4G4
• Molecular Weight: 425,137 Da
• NCBI Official Full Name: Alstrom syndrome protein 1
• NCBI Official Synonym Full Names: Alstrom syndrome protein 1
• NCBI Official Symbol: ALMS1
• NCBI Official Synonym Symbols: ALSS
• NCBI Protein Information: Alstrom syndrome protein 1
• UniProt Protein Name: Alstrom syndrome protein 1
• Protein Family: Alstrom syndrome protein
• UniProt Gene Name: ALMS1
• UniProt Synonym Gene Names: KIAA0328
• UniProt Entry Name: ALMS1_HUMAN

NCBI Description

This gene encodes a protein containing a large tandem-repeat domain as well as additional low complexity regions. The encoded protein functions in microtubule organization, particularly in the formation and maintanance of cilia. Mutations in this gene cause Alstrom syndrome. There is a pseudogene for this gene located adjacent in the same region of chromosome 2. Alternative splice variants have been described but their full length nature has not been determined. [provided by RefSeq, Apr 2014]

Uniprot Description

ALMS1: Involved in PCM1-dependent intracellular transport. Required, directly or indirectly, for the localization of NCAPD2 to the proximal ends of centrioles. Required for proper formation and/or maintenance of primary cilia (PC), microtubule-based structures that protrude from the surface of epithelial cells. Defects in ALMS1 are the cause of Alstrom syndrome (ALMS). Alstrom syndrome is a rare autosomal recessive disorder characterized by progressive cone-rod retinal dystrophy, neurosensory hearing loss, early childhood obesity and type 2 diabetes mellitus. Dilated cardiomyopathy, acanthosis nigricans, male hypogonadism, hypothyroidism, developmental delay and hepatic dysfunction can also be associated with the syndrome. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation

Chromosomal Location of Human Ortholog: 2p13

Cellular Component: centriole; nucleoplasm; spindle pole; centrosome; cytoplasm; cytosol; nucleus; cilium

Molecular Function: protein binding

Biological Process: organelle organization and biogenesis; regulation of stress fiber formation; mitotic cell cycle; G2/M transition of mitotic cell cycle; endosome transport

Disease: Alstrom Syndrome