ALKBH8 sirna

ALKBH8 siRNA (Human)

Cat. Num. AAA8239507

• Gene Names: ALKBH8; ABH8; TRM9; TRMT9
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ALKBH8; ALKBH8 siRNA (Human); ABH8; Alkylated DNA repair protein alkB homolog 8; Probable alpha-ketoglutarate-dependent dioxygenase ABH8; S-adenosyl-L-methionine-dependent tRNA methyltransferase ABH8; tRNA; carboxymethyluridine(34)-5-O)-methyltransferase ABH8; ALKBH8 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ALKBH8 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 667

• Applicable Applications for ALKBH8 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ALKBH8 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ALKBH8 sirna

siRNA to inhibit ALKBH8 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 666335573
• NCBI GeneID: 91801
• NCBI Accession #: NP_001287939.1
• NCBI GenBank Nucleotide #: NM_001301010.1
• UniProt Accession #: Q96BT7; Q8N989; B1Q2M0; B4DEF6
• Molecular Weight: 75,558 Da
• NCBI Official Full Name: alkylated DNA repair protein alkB homolog 8 isoform 1
• NCBI Official Synonym Full Names: alkB homolog 8, tRNA methyltransferase
• NCBI Official Symbol: ALKBH8
• NCBI Official Synonym Symbols: ABH8; TRM9; TRMT9
• NCBI Protein Information: alkylated DNA repair protein alkB homolog 8
• UniProt Protein Name: Alkylated DNA repair protein alkB homolog 8
• Protein Family: Alkylated DNA repair protein
• UniProt Gene Name: ALKBH8
• UniProt Synonym Gene Names: ABH8
• UniProt Entry Name: ALKB8_HUMAN

Uniprot Description

ALKBH8: Catalyzes the methylation of 5-carboxymethyl uridine to 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in tRNA. Catalyzes the last step in the formation of 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in target tRNA. Has a preference for tRNA(Arg) and tRNA(Glu), and does not bind tRNA(Lys). Required for normal survival after DNA damage. May inhibit apoptosis and promote cell survival and angiogenesis. Belongs to the alkB family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Methyltransferase; RNA processing; EC 2.1.1.229

Chromosomal Location of Human Ortholog: 11q22.3

Cellular Component: nucleoplasm; microtubule cytoskeleton; nucleus; cytosol

Molecular Function: protein binding; zinc ion binding; tRNA (uracil) methyltransferase activity; iron ion binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors; tRNA binding

Biological Process: tRNA wobble uridine modification; response to DNA damage stimulus; tRNA methylation