AKAP9 sirna

AKAP9 siRNA (Human)

Cat. Num. AAA8230996

• Gene Names: AKAP9; LQT11; PRKA9; AKAP-9; CG-NAP; YOTIAO; AKAP350; AKAP450; PPP1R45; HYPERION; MU-RMS-40.16A
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: AKAP9; AKAP9 siRNA (Human); AKAP350; AKAP450; KIAA0803; A-kinase anchor protein 9; AKAP-9; A-kinase anchor protein 350 kDa; AKAP 350; hgAKAP 350; A-kinase anchor protein 450 kDa; AKAP 450; AKAP 120-like protein; Centrosome- and Golgi-localized PKN-associated protein; CG-NAP; Protein hyperion; Protein kinase A-anchoring protein 9; PRKA9; Protein yotiao; AKAP9 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: AKAP9 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 3,929

• Applicable Applications for AKAP9 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human AKAP9 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for AKAP9 sirna

siRNA to inhibit AKAP9 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 22538387
• NCBI GeneID: 10142
• NCBI Accession #: NP_005742.4
• NCBI GenBank Nucleotide #: NM_005751.4
• UniProt Accession #: Q99996; O14869; O43355; O94895; Q75N20; Q9UQH3; Q9UQQ4; Q9Y6B8; Q9Y6Y2; A4D1F0; A4D1F2; A4D1F4
• Molecular Weight: 455,423 Da
• NCBI Official Full Name: A-kinase anchor protein 9 isoform 2
• NCBI Official Synonym Full Names: A kinase (PRKA) anchor protein 9
• NCBI Official Symbol: AKAP9
• NCBI Official Synonym Symbols: LQT11; PRKA9; AKAP-9; CG-NAP; YOTIAO; AKAP350; AKAP450; PPP1R45; HYPERION; MU-RMS-40.16A
• NCBI Protein Information: A-kinase anchor protein 9
• UniProt Protein Name: A-kinase anchor protein 9
• Protein Family: A-kinase anchor protein
• UniProt Gene Name: AKAP9
• UniProt Synonym Gene Names: AKAP350; AKAP450; KIAA0803; AKAP-9; AKAP 350; hgAKAP 350; AKAP 450; CG-NAP; PRKA9
• UniProt Entry Name: AKAP9_HUMAN

NCBI Description

The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. Alternate splicing of this gene results in at least two isoforms that localize to the centrosome and the Golgi apparatus, and interact with numerous signaling proteins from multiple signal transduction pathways. These signaling proteins include type II protein kinase A, serine/threonine kinase protein kinase N, protein phosphatase 1, protein phosphatase 2a, protein kinase C-epsilon and phosphodiesterase 4D3. [provided by RefSeq, Aug 2008]

Uniprot Description

AKAP9: Binds to type II regulatory subunits of protein kinase A. Scaffolding protein that assembles several protein kinases and phosphatases on the centrosome and Golgi apparatus. May be required to maintain the integrity of the Golgi apparatus. Isoform 4 is associated with the N-methyl-D-aspartate receptor and is specifically found in the neuromuscular junction (NMJ) as well as in neuronal synapses, suggesting a role in the organization of postsynaptic specializations. Defects in AKAP9 are the cause of long QT syndrome type 11 (LQT11). Long QT syndromes are heart disorders characterized by a prolonged QT interval on the ECG and polymorphic ventricular arrhythmias. They cause syncope and sudden death in response to excercise or emotional stress. They can present with a sentinel event of sudden cardiac death in infancy. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 7q21-q22

Cellular Component: Golgi apparatus; centrosome; voltage-gated potassium channel complex; pericentriolar material; cytoskeleton; intracellular membrane-bound organelle; cytosol

Molecular Function: protein binding; potassium channel regulator activity; protein complex scaffold; receptor binding

Biological Process: synaptic transmission; transport; organelle organization and biogenesis; spermatogenesis; mitotic cell cycle; G2/M transition of mitotic cell cycle; signal transduction; positive regulation of peptidyl-serine phosphorylation; Sertoli cell development

Disease: Long Qt Syndrome 11