AIFM1 sirna

AIFM1 siRNA (Human)

Cat. Num. AAA8231709

• Gene Names: AIFM1; AIF; CMT2D; CMTX4; COWCK; NADMR; NAMSD; PDCD8; COXPD6
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: AIFM1; AIFM1 siRNA (Human); AIF; PDCD8; Apoptosis-inducing factor 1 mitochondrial; Programmed cell death protein 8; AIFM1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: AIFM1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 261

• Applicable Applications for AIFM1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human AIFM1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for AIFM1 sirna

siRNA to inhibit AIFM1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 195927004
• NCBI GeneID: 9131
• NCBI Accession #: NP_001124318.1
• NCBI GenBank Nucleotide #: NM_001130846.2
• UniProt Accession #: O95831; Q1L6K4; Q1L6K6; Q2QKE4; Q5JUZ7; Q6I9X6; Q9Y3I3; Q9Y3I4; A4QPB4; B1ALN1; B2RB08; D3DTE9
• Molecular Weight: 26,033 Da
• NCBI Official Full Name: apoptosis-inducing factor 1, mitochondrial isoform 4
• NCBI Official Synonym Full Names: apoptosis-inducing factor, mitochondrion-associated, 1
• NCBI Official Symbol: AIFM1
• NCBI Official Synonym Symbols: AIF; CMT2D; CMTX4; COWCK; NADMR; NAMSD; PDCD8; COXPD6
• NCBI Protein Information: apoptosis-inducing factor 1, mitochondrial
• UniProt Protein Name: Apoptosis-inducing factor 1, mitochondrial
• UniProt Gene Name: AIFM1
• UniProt Synonym Gene Names: AIF; PDCD8
• UniProt Entry Name: AIFM1_HUMAN

NCBI Description

This gene encodes a flavoprotein essential for nuclear disassembly in apoptotic cells, and it is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it affects chromosome condensation and fragmentation. In addition, this gene product induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Mutations in this gene cause combined oxidative phosphorylation deficiency 6 (COXPD6), a severe mitochondrial encephalomyopathy, as well as Cowchock syndrome, also known as X-linked recessive Charcot-Marie-Tooth disease-4 (CMTX-4), a disorder resulting in neuropathy, and axonal and motor-sensory defects with deafness and mental retardation. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 10. [provided by RefSeq, Aug 2015]

Uniprot Description

PDCD8: Probable oxidoreductase that has a dual role in controlling cellular life and death; during apoptosis, it is translocated from the mitochondria to the nucleus to function as a proapoptotic factor in a caspase-independent pathway, while in normal mitochondria, it functions as an antiapoptotic factor via its oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e., caspase-independent fragmentation of chromosomal DNA. Interacts with EIF3G,and thereby inhibits the EIF3 machinery and protein synthesis, and activates casapse-7 to amplify apoptosis. Plays a critical role in caspase- independent, pyknotic cell death in hydrogen peroxide-exposed cells. Binds to DNA in a sequence-independent manner. Interacts with XIAP/BIRC4. Interacts (via N-terminus) with EIF3G (via C-terminus). Belongs to the FAD-dependent oxidoreductase family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial; EC 1.-.-.-; Oxidoreductase; Apoptosis

Chromosomal Location of Human Ortholog: Xq26.1

Cellular Component: mitochondrion; perinuclear region of cytoplasm; mitochondrial inner membrane; mitochondrial intermembrane space; cytosol; nucleus

Molecular Function: protein dimerization activity; oxidoreductase activity, acting on NADH or NADPH; protein binding; DNA binding; electron carrier activity; NAD(P)H oxidase activity

Biological Process: neuron differentiation; chromosome condensation; caspase activation; mitochondrial respiratory chain complex I assembly; neuron apoptosis; cell redox homeostasis; apoptosis; positive regulation of apoptosis; DNA fragmentation during apoptosis; DNA catabolic process

Disease: Cowchock Syndrome