AHNAK sirna

AHNAK siRNA (Human)

Cat. Num. AAA8232453

• Gene Names: AHNAK; AHNAKRS
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: AHNAK; AHNAK siRNA (Human); PM227; Neuroblast differentiation-associated protein AHNAK; Desmoyokin; AHNAK sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: AHNAK siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 5890

• Applicable Applications for AHNAK sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human AHNAK gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for AHNAK sirna

siRNA to inhibit AHNAK expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 61743954
• NCBI GeneID: 79026
• NCBI Accession #: NP_001611.1
• NCBI GenBank Nucleotide #: NM_001620.2
• UniProt Accession #: Q09666; A1A586
• Molecular Weight: 16,061 Da
• NCBI Official Full Name: neuroblast differentiation-associated protein AHNAK isoform 1
• NCBI Official Synonym Full Names: AHNAK nucleoprotein
• NCBI Official Symbol: AHNAK
• NCBI Official Synonym Symbols: AHNAKRS
• NCBI Protein Information: neuroblast differentiation-associated protein AHNAK
• UniProt Protein Name: Neuroblast differentiation-associated protein AHNAK
• Protein Family: Neuroblast differentiation-associated protein
• UniProt Gene Name: AHNAK
• UniProt Synonym Gene Names: PM227
• UniProt Entry Name: AHNK_HUMAN

Uniprot Description

AHNAK: a giant propeller-like protein that associates with calcium channel proteins of cardiomyocytes and other cells. Translocates from the cytosol to the plasma membrane during the formation of cell-cell contacts and the development of epithelial polarity. At the plasma membrane, it associates as a multimeric complex with actin and the annexin 2/S100A10 complex. The AHNAK/annexin 2/S100A10 complex regulates cortical actin cytoskeleton organization and the architecture of the cell membrane.

Protein type: Actin-binding; Adaptor/scaffold; Cytoskeletal

Chromosomal Location of Human Ortholog: 11q12.2

Cellular Component: actin cytoskeleton; cell-cell adherens junction; costamere; cytoplasm; cytosol; focal adhesion; lipid raft; lysosomal membrane; membrane; nucleus; plasma membrane; sarcolemma; vesicle

Molecular Function: protein binding

Biological Process: protein oligomerization; regulation of RNA splicing