ADH5 sirna

ADH5 siRNA (Human)

Cat. Num. AAA8206345

• Gene Names: ADH5; FDH; ADHX; ADH-3; FALDH; GSNOR; GSH-FDH
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ADH5; ADH5 siRNA (Human); ADHX; FDH; Alcohol dehydrogenase class-3; Alcohol dehydrogenase 5; Alcohol dehydrogenase class chi chain; Alcohol dehydrogenase class-III; Glutathione-dependent formaldehyde dehydrogenase; FALDH; GSH-FDH; S-(hydroxymethyl)glutathione dehydrogenase; ADH5 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ADH5 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 374

• Applicable Applications for ADH5 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ADH5 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ADH5 sirna

siRNA to inhibit ADH5 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 71565154
• NCBI GeneID: 128
• NCBI Accession #: NP_000662.3
• NCBI GenBank Nucleotide #: NM_000671.4
• UniProt Accession #: P11766; Q6FHR2
• Molecular Weight: 39,724 Da
• NCBI Official Full Name: alcohol dehydrogenase class-3
• NCBI Official Synonym Full Names: alcohol dehydrogenase 5 (class III), chi polypeptide
• NCBI Official Symbol: ADH5
• NCBI Official Synonym Symbols: FDH; ADHX; ADH-3; FALDH; GSNOR; GSH-FDH
• NCBI Protein Information: alcohol dehydrogenase class-3
• UniProt Protein Name: Alcohol dehydrogenase class-3
• Protein Family: Alcohol dehydrogenase
• UniProt Gene Name: ADH5
• UniProt Synonym Gene Names: FALDH; FDH; GSH-FDH
• UniProt Entry Name: ADHX_HUMAN

NCBI Description

This gene encodes a member of the alcohol dehydrogenase family. Members of this family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. The encoded protein forms a homodimer. It has virtually no activity for ethanol oxidation, but exhibits high activity for oxidation of long-chain primary alcohols and for oxidation of S-hydroxymethyl-glutathione, a spontaneous adduct between formaldehyde and glutathione. This enzyme is an important component of cellular metabolism for the elimination of formaldehyde, a potent irritant and sensitizing agent that causes lacrymation, rhinitis, pharyngitis, and contact dermatitis. The human genome contains several non-transcribed pseudogenes related to this gene. [provided by RefSeq, Oct 2008]

Uniprot Description

ADH5: Class-III ADH is remarkably ineffective in oxidizing ethanol, but it readily catalyzes the oxidation of long-chain primary alcohols and the oxidation of S-(hydroxymethyl) glutathione. Belongs to the zinc-containing alcohol dehydrogenase family. Class-III subfamily.

Protein type: Xenobiotic Metabolism - metabolism by cytochrome P450; Amino Acid Metabolism - tyrosine; EC 1.1.1.1; Mitochondrial; Lipid Metabolism - fatty acid; EC 1.1.1.284; Cofactor and Vitamin Metabolism - retinol; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Oxidoreductase; Energy Metabolism - methane

Chromosomal Location of Human Ortholog: 4q23

Cellular Component: mitochondrion; nucleus

Molecular Function: protein homodimerization activity; electron carrier activity; zinc ion binding; formaldehyde dehydrogenase activity; alcohol dehydrogenase activity; S-(hydroxymethyl)glutathione dehydrogenase activity; fatty acid binding

Biological Process: response to nitrosative stress; respiratory system process; ethanol catabolic process; formaldehyde catabolic process; peptidyl-cysteine S-nitrosylation; response to redox state; response to lipopolysaccharide; ethanol oxidation; retinoid metabolic process; positive regulation of blood pressure; aging