ACKR2 sirna

ACKR2 siRNA (Human)

Cat. Num. AAA8235876

• Gene Names: ACKR2; D6; hD6; CCR9; CCBP2; CCR10; CMKBR9
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ACKR2; ACKR2 siRNA (Human); CCBP2; CCR10; CMKBR9; D6; Atypical chemokine receptor 2; C-C chemokine receptor D6; Chemokine receptor CCR-10; Chemokine receptor CCR-9; Chemokine-binding protein 2; Chemokine-binding protein D6; ACKR2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ACKR2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 384

• Applicable Applications for ACKR2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ACKR2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ACKR2 sirna

siRNA to inhibit ACKR2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 13929467
• NCBI GeneID: 1238
• NCBI Accession #: NP_001287.2
• NCBI GenBank Nucleotide #: NM_001296.4
• UniProt Accession #: O00590; O00537; Q53YA1; Q86UN9; Q96A02; B2R8Y8
• Molecular Weight: 43,443 Da
• NCBI Official Full Name: atypical chemokine receptor 2
• NCBI Official Synonym Full Names: atypical chemokine receptor 2
• NCBI Official Symbol: ACKR2
• NCBI Official Synonym Symbols: D6; hD6; CCR9; CCBP2; CCR10; CMKBR9
• NCBI Protein Information: atypical chemokine receptor 2
• UniProt Protein Name: Atypical chemokine receptor 2
• Protein Family: Atypical chemokine receptor
• UniProt Gene Name: ACKR2
• UniProt Synonym Gene Names: CCBP2; CCR10; CMKBR9; D6
• UniProt Entry Name: ACKR2_HUMAN

NCBI Description

This gene encodes a beta chemokine receptor, which is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. Chemokines and their receptor-mediated signal transduction are critical for the recruitment of effector immune cells to the inflammation site. This gene is expressed in a range of tissues and hemopoietic cells. The expression of this receptor in lymphatic endothelial cells and overexpression in vascular tumors suggested its function in chemokine-driven recirculation of leukocytes and possible chemokine effects on the development and growth of vascular tumors. This receptor appears to bind the majority of beta-chemokine family members; however, its specific function remains unknown. This gene is mapped to chromosome 3p21.3, a region that includes a cluster of chemokine receptor genes. [provided by RefSeq, Jul 2008]

Uniprot Description

CCBP2: Receptor for C-C type chemokines including SCYA2/MCP-1, SCY3/MIP-1-alpha, SCYA5/RANTES and SCYA7/MCP-3. Belongs to the G-protein coupled receptor 1 family.

Protein type: GPCR, family 1; Receptor, GPCR; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 3p21.3

Cellular Component: recycling endosome; integral to plasma membrane; early endosome; plasma membrane; actin filament

Molecular Function: C-C chemokine receptor activity; chemokine receptor activity; C-C chemokine binding; scavenger receptor activity; C-X-C chemokine receptor activity

Biological Process: receptor-mediated endocytosis; G-protein coupled receptor protein signaling pathway; neutrophil activation; multicellular organismal development; immune response; chemotaxis; inflammatory response