ABCB4 sirna

ABCB4 siRNA (Human)

Cat. Num. AAA8200937

• Gene Names: ABCB4; GBD1; ICP3; MDR2; MDR3; PGY3; ABC21; MDR2/3; PFIC-3
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ABCB4; ABCB4 siRNA (Human); MDR3; PGY3; Multidrug resistance protein 3; ATP-binding cassette sub-family B member 4; P-glycoprotein 3; ABCB4 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ABCB4 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1,232

• Applicable Applications for ABCB4 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ABCB4 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ABCB4 sirna

siRNA to inhibit ABCB4 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 4505771
• NCBI GeneID: 5244
• NCBI Accession #: NP_000434.1
• NCBI GenBank Nucleotide #: NM_000443.3
• UniProt Accession #: P21439; Q14813; A0A2V7; A4D1D3; A4D1D4; A4D1D5; D6W5P3; D6W5P4
• Molecular Weight: 135,259 Da
• NCBI Official Full Name: multidrug resistance protein 3 isoform A
• NCBI Official Synonym Full Names: ATP-binding cassette, sub-family B (MDR/TAP), member 4
• NCBI Official Symbol: ABCB4
• NCBI Official Synonym Symbols: GBD1; ICP3; MDR2; MDR3; PGY3; ABC21; MDR2/3; PFIC-3
• NCBI Protein Information: multidrug resistance protein 3
• UniProt Protein Name: Multidrug resistance protein 3
• Protein Family: ABC transporter B family
• UniProt Gene Name: ABCB4
• UniProt Synonym Gene Names: MDR3; PGY3
• UniProt Entry Name: MDR3_HUMAN

NCBI Description

The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance as well as antigen presentation. This gene encodes a full transporter and member of the p-glycoprotein family of membrane proteins with phosphatidylcholine as its substrate. The function of this protein has not yet been determined; however, it may involve transport of phospholipids from liver hepatocytes into bile. Alternative splicing of this gene results in several products of undetermined function. [provided by RefSeq, Jul 2008]

Uniprot Description

ABCB4: a transporter that mediates ATP-dependent export of organic anions and drugs from the cytoplasm. Hydrolyzes ATP with low efficiency. Human ABCB4 is not capable of conferring drug resistance. Mediates the translocation of phosphatidylcholine across the canalicular membrane of the hepatocyte. Interacts with HAX1. Defects are the cause of gallstones, and progressive familial intrahepatic cholestasis 3, an autosomal recessive liver disorder that progresses to cirrhosis and liver failure before adulthood. Two alternatively spliced human isoforms have been described.

Protein type: Hydrolase; Transporter; Transporter, ABC family; Membrane protein, multi-pass; Membrane protein, integral; EC 3.6.3.44

Chromosomal Location of Human Ortholog: 7q21.1

Cellular Component: intercellular canaliculus; membrane; integral to plasma membrane; integral to membrane; plasma membrane

Molecular Function: ATPase activity, coupled to transmembrane movement of substances; ATP binding; xenobiotic-transporting ATPase activity

Biological Process: response to drug; transport; multidrug transport; xenobiotic transport; lipid metabolic process; cellular lipid metabolic process; transmembrane transport

Disease: Gallbladder Disease 1; Cholestasis, Progressive Familial Intrahepatic, 3; Cholestasis, Intrahepatic, Of Pregnancy 3