CENPA blocking peptide

CENPA Blocking Peptide

Cat. Num. AAA8230985

• Gene Names: CENPA; CenH3; CENP-A
• Reactivity: Human, Mouse, Rat
• Applications: Blocking (BL)
• Purity: >85%
• Synonyms: CENPA; CENPA Blocking Peptide; Histone H3-like centromeric protein A; Centromere autoantigen A; Centromere protein A; CENP-A; CENPA blocking peptide

• Host: Synthetic
• Reactivity: Human, Mouse, Rat
• Purity/Purification: >85%
• Form/Format: Lyophilized powder
• Sequence Length: 114

• Applicable Applications for CENPA blocking peptide: Blocking (BL)
• Quality Control: The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry.
• Directions for Use: Blocking Peptide to the diluted primary antibody in a molar ratio of 10:1 (peptide to antibody) and incubate the mixture at 4 degree C for overnight or at room temperature for 2 hours.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CENPA blocking peptide

The peptide is used to block Anti-CENPA Antibody reactivity.

NCBI and Uniprot Product Information

• NCBI GI #: 109637779
• NCBI GeneID: 1058
• NCBI Accession #: NP_001035891.1
• NCBI GenBank Nucleotide #: NM_001042426.1
• UniProt Accession #: P49450; Q53T74; Q9BVW2; D6W544
• Molecular Weight: 13,001 Da
• NCBI Official Full Name: histone H3-like centromeric protein A isoform b
• NCBI Official Synonym Full Names: centromere protein A
• NCBI Official Symbol: CENPA
• NCBI Official Synonym Symbols: CenH3; CENP-A
• NCBI Protein Information: histone H3-like centromeric protein A
• UniProt Protein Name: Histone H3-like centromeric protein A
• Protein Family: Histone H3-like centromeric protein
• UniProt Gene Name: CENPA
• UniProt Synonym Gene Names: CENP-A
• UniProt Entry Name: CENPA_HUMAN

NCBI Description

Centromeres are the differentiated chromosomal domains that specify the mitotic behavior of chromosomes. CENPA encodes a centromere protein which contains a histone H3 related histone fold domain that is required for targeting to the centromere. CENPA is proposed to be a component of a modified nucleosome or nucleosome-like structure in which it replaces 1 or both copies of conventional histone H3 in the (H3-H4)2 tetrameric core of the nucleosome particle. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

CENPA: a basic nuclear protein of the histone H3 family. May act as a core histone necessary for the assembly of centromeres. Contains a histone H3 related histone fold domain that is required for targeting to the centromere. CENPA is proposed to be a component of a modified nucleosome or nucleosome-like structure in which it replaces 1 or both copies of conventional histone H3 in the (H3-H4)2 tetrameric core of the nucleosome particle.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 2p23.3

Cellular Component: nucleoplasm; inner kinetochore of condensed chromosome; nucleosome; nucleus; cytosol; chromosome, pericentric region; condensed nuclear chromosome, pericentric region

Molecular Function: protein binding; DNA binding; protein heterodimerization activity; chromatin binding

Biological Process: nucleosome assembly; kinetochore assembly; DNA replication-independent nucleosome assembly at centromere; viral reproduction; establishment of mitotic spindle orientation; mitotic cell cycle