SMUG1 blocking peptide

SMUG1 Blocking Peptide

Cat. Num. AAA823125

• Gene Names: SMUG1; FDG; UNG3; HMUDG
• Reactivity: Human, Mouse, Rat, Monkey
• Applications: Blocking (BL)
• Purity: >85%
• Synonyms: SMUG1; SMUG1 Blocking Peptide; Single-strand selective monofunctional uracil DNA glycosylase; SMUG1 blocking peptide

• Host: Synthetic
• Reactivity: Human, Mouse, Rat, Monkey
• Purity/Purification: >85%
• Form/Format: Lyophilized powder
• Sequence Length: 270

• Applicable Applications for SMUG1 blocking peptide: Blocking (BL)
• Application Notes: Blocking Peptide to the diluted primary antibody in a molar ratio of 10:1 (peptide to antibody) and incubate the mixture at 4 degree C for overnight or at room temperature for 2 hours.
• Quality Control: The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SMUG1 blocking peptide

The peptide is used to block Anti-SMUG1 Antibody reactivity.

NCBI and Uniprot Product Information

• NCBI GI #: 344925869
• NCBI GeneID: 23583
• NCBI Accession #: NP_001230716.1
• NCBI GenBank Nucleotide #: NM_001243787.1
• UniProt Accession #: Q53HV7; O95862; Q0D2M0; Q8NB71; Q9BWC8; A8K2K9
• Molecular Weight: 19,627 Da
• NCBI Official Full Name: single-strand selective monofunctional uracil DNA glycosylase isoform 1
• NCBI Official Synonym Full Names: single-strand-selective monofunctional uracil-DNA glycosylase 1
• NCBI Official Symbol: SMUG1
• NCBI Official Synonym Symbols: FDG; UNG3; HMUDG
• NCBI Protein Information: single-strand selective monofunctional uracil DNA glycosylase
• UniProt Protein Name: Single-strand selective monofunctional uracil DNA glycosylase
• Protein Family: Single-strand selective monofunctional uracil DNA glycosylase
• UniProt Gene Name: SMUG1
• UniProt Entry Name: SMUG1_HUMAN

NCBI Description

This gene encodes a protein that participates in base excision repair by removing uracil from single- and double-stranded DNA. Many alternatively spliced transcript variants exist for this gene; the full-length nature is known for some but not all of the variants. [provided by RefSeq, Aug 2011]

Uniprot Description

SMUG1: Responsible for recognizing base lesions in the genome and initiating base excision DNA repair. Acts as a monofunctional DNA glycosylase specific for uracil (U) residues in DNA and has a preference for single-stranded DNA substrates. The activity is greater against mismatches (U/G) than against matches (U/A). Excised uracil (U), 5-formyluracil (fU) and uracil derivatives bearing an oxidized group at C5 [5-hydroxyuracil (hoU) and 5- hydroxymethyluracil (hmU)] in ssDNA and dsDNA but not analogous cytosine derivatives (5-hydroxycytosine and 5-formylcytosine) and other oxidized damage. The activity is damage specificity and salt concentration-dependent. The general order of the preference for ssDNA and dsDNA is the following: ssDNA > dsDNA (G pair) = dsDNA (A pair) at the low salt concentration. At the high concentration dsDNA (G pair) > dsDNA (A pair) > ssDNA. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA repair, damage; Hydrolase; EC 3.2.2.-

Chromosomal Location of Human Ortholog: 12q13.13

Cellular Component: nucleoplasm; intracellular membrane-bound organelle; nucleolus

Molecular Function: single-strand selective uracil DNA N-glycosylase activity; protein binding; uracil DNA N-glycosylase activity; DNA binding; oxidized pyrimidine base lesion DNA N-glycosylase activity; DNA N-glycosylase activity

Biological Process: base-excision repair, AP site formation; base-excision repair; depyrimidination; DNA repair; DNA catabolic process, endonucleolytic