Superoxide Dismutase 1 Recombinant Protein | SOD1 recombinant protein
Recombinant Superoxide Dismutase 1, Soluble (SOD1)
Applications
SDS-Page, Western Blot, ELISA, Immunoprecipitation
Purity
>95%
Synonyms
Superoxide Dismutase 1; Recombinant Superoxide Dismutase 1; Soluble (SOD1); SOD1 recombinant protein
Host
Host: E Coli
Source: Prokaryotic expression
Source: Prokaryotic expression
Purity/Purification
>95%
Form/Format
Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Concentration
6.7 (varies by lot)
Sequence
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- ATKAVCVLK GDGPVQGTIR FEAKGDKVVV TGSITGLTEG DHGFHVHQFG DNTQGCTSAG PHFNPLSKKH GGPKDEERHV GDLGNVKADK NGVAIVDIVD PLISLSGEYS IIGRTMVVHE KPDDLGRGGN EESTKTGNAG GRLACGVIGI AP
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- ATKAVCVLK GDGPVQGTIR FEAKGDKVVV TGSITGLTEG DHGFHVHQFG DNTQGCTSAG PHFNPLSKKH GGPKDEERHV GDLGNVKADK NGVAIVDIVD PLISLSGEYS IIGRTMVVHE KPDDLGRGGN EESTKTGNAG GRLACGVIGI AP
Applicable Applications for SOD1 recombinant protein
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
Predicted Molecular Mass
19.4kDa
Accurate Molecular Mass (KD)
19kDa
Endotoxin
<1.0EU per 1ug (determined by the LAL method)
Expression System
Prokaryotic expression
Tag
two N-terminal Tags, His-tag and T7-tag
Organism Species
Ovis aries; Ovine (Sheep)
Fragment
Ala2~Pro152
Usage
Reconstitute in sterile PBS, pH7.2-pH7.4.
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
SDS-Page
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Product Notes
The SOD1 (Catalog #AAA2031341) is a Recombinant Protein produced from Host: E Coli Source: Prokaryotic expression and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Superoxide Dismutase 1 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). Researchers should empirically determine the suitability of the SOD1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.MGSSHHHHH H SSGLVPRGSH MASMTGGQQM GRGSEF- ATKAVCVLK GDGPVQGTIR FEAKGDKVVV TGSITGLTEG DHGFHVHQFG DNTQGCTSAG PHFNPLSKKH GGPKDEERHV GDLGNVKADK NGVAIVDIVD PLISLSGEYS IIGRTMVVHE KPDDLGRGGN EESTKTGNAG GRLACGVIGI AP
