Human sulfatase 1 ELISA Kit | SULF1 elisa kit

Human Extracellular sulfatase Sulf-1, SULF1 ELISA Kit

Cat. Num. AAA943783

• Gene Names: SULF1; SULF-1; HSULF-1
• Reactivity: Human
• Synonyms: sulfatase 1; Human Extracellular sulfatase Sulf-1; SULF1 ELISA Kit; Human Extracellular sulfatase Sulf-1 (SULF1) ELISA kit; FLJ30905; FLJ38022; FLJ41750; HSULF-1; KIAA1077; SULF-1; sulfatase FP; SULF1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human SULF1. No significant cross-reactivity or interference between human SULF1 and analogues was observed.
• Sequence Length: 871

• Samples: Serum, plasma, tissue homogenates, cell lysates
• Assay Type: Quantitative Sandwich
• Detection Range: 7.8 pg/ml-500 pg/ml
• Sensitivity: < 1.95 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SULF1 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SULF1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SULF1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SULF1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SULF1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 189571638
• NCBI GeneID: 23213
• NCBI Accession #: NP_001121676.1
• NCBI GenBank Nucleotide #: NM_001128204.1
• UniProt Accession #: Q8IWU6; Q86YV8; Q8NCA2; Q9UPS5
• Molecular Weight: 101,027 Da
• NCBI Official Full Name: extracellular sulfatase Sulf-1
• NCBI Official Synonym Full Names: sulfatase 1
• NCBI Official Symbol: SULF1
• NCBI Official Synonym Symbols: SULF-1; HSULF-1
• NCBI Protein Information: extracellular sulfatase Sulf-1; sulfatase FP
• UniProt Protein Name: Extracellular sulfatase Sulf-1
• Protein Family: Extracellular sulfatase
• UniProt Gene Name: SULF1
• UniProt Synonym Gene Names: KIAA1077; hSulf-1
• UniProt Entry Name: SULF1_HUMAN

NCBI Description

This gene encodes an extracellular heparan sulfate endosulfatase. The encoded enzyme selectively removes 6-O-sulfate groups from heparan sulfate chains of heparan sulfate proteoglycans (HSPGs). The enzyme is secreted through the Golgi and is subsequently localized to the cell surface. The expression of this gene may be down-regulated in several types of cancer, including hepatocellular (HCC), ovarian and breast cancers. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]

Uniprot Description

SULF1: Exhibits arylsulfatase activity and highly specific endoglucosamine-6-sulfatase activity. It can remove sulfate from the C-6 position of glucosamine within specific subregions of intact heparin. Diminishes HSPG (heparan sulfate proteoglycans) sulfation, inhibits signaling by heparin-dependent growth factors, diminishes proliferation, and facilitates apoptosis in response to exogenous stimulation. Belongs to the sulfatase family.

Protein type: Hydrolase; EC 3.1.6.-; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 8q13.2

Cellular Component: Golgi apparatus; extracellular space; Golgi stack; cell surface; endoplasmic reticulum; plasma membrane; lipid raft

Molecular Function: arylsulfatase activity; N-acetylglucosamine-6-sulfatase activity; calcium ion binding

Biological Process: negative regulation of fibroblast growth factor receptor signaling pathway; apoptosis; embryonic skeletal development; glomerular basement membrane development; glomerular filtration; regulation of fibroblast growth factor receptor signaling pathway; chondrocyte development; negative regulation of angiogenesis; cartilage development; esophagus smooth muscle contraction; negative regulation of endothelial cell proliferation; heparan sulfate proteoglycan metabolic process; kidney development; vascular endothelial growth factor receptor signaling pathway; negative regulation of cell migration; positive regulation of Wnt receptor signaling pathway; positive regulation of BMP signaling pathway