Streptavidin Protein

Streptavidin (Sepharose 4B)

Applications

Immunoprecipitation

Purity

Purified

Synonyms

Streptavidin; Streptavidin (Sepharose 4B); Streptavidin protein

Ordering
Cat. Num. AAA634542 When autocomplete results are available use up and down arrows to review and enter to select. Touch device users, explore by touch or with swipe gestures. For Research Use Only!

Host

Streptocyces avidinii

Purity/Purification

Purified

Form/Format

Supplied as a liquid in 50% suspension in 10mM PBS, pH 7.4 with 0.1% proclin

Applicable Applications for Streptavidin protein

Immunoprecipitation (IP)

Application Notes

Suitable for use in Immunoprecipitation.
Dilution: Immunoprecipitation: 50-100ul of 10% suspension for each sample. Upon boiling, may denature to show four subunits of ~15kD each.

Immunogen

Streptavidin (Streptomyces avidinii), a biotin binding protein with exceptionally strong binding affinity, is covalently coupled to Sepharose 4B

Binding capacity

> 300nmol biotin/ml medium;
6mg biotinylated BSA/ml medium

Preparation and Storage

Store in 20% ethanol. May be stored at 4 degree C. Do not freeze. Stable for at least 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Related Product Information for Streptavidin protein

Streptavidin (MW ~66kD) binds specifically with biotin (244D). It is derived from the bacterium Streptomyces avidinii and bears a remarkable similarity to chicken egg-white avidin both in three-dimensional structure and its ability to bind biotin with extremely high affinity (Kd=10e-15M). It is a tetrameric protein capable of binding up to 4 biotin molecules. Unlike avidin, Streptavidin is non-glycosylated and is essentially neutral in charge, whereas avidin (pH 10.5) is basic at neutral pH. Because of this, streptavidin has considerably less non-specific binding resulting in less background. It has replaced avidin as the reagent of choice for most applications where protein interactions may cause background.

Product Categories/Family for Streptavidin protein

Molecular Biology; MB-Proteins

NCBI and Uniprot Product Information

NCBI GI #

21340185

NCBI Official Full Name

streptavidin

Protein Family

Streptavidin

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Product Notes

The Streptavidin (Catalog #AAA634542) is a Protein produced from Streptocyces avidinii and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Streptavidin can be used in a range of immunoassay formats including, but not limited to, Immunoprecipitation (IP). Suitable for use in Immunoprecipitation. Dilution: Immunoprecipitation: 50-100ul of 10% suspension for each sample. Upon boiling, may denature to show four subunits of ~15kD each. Researchers should empirically determine the suitability of the Streptavidin for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Streptavidin, Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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